| 引用本文: |
姜艳玲,王金华,李发余,徐善荣,郭海龙.丹白颗粒对子宫内膜异位症大鼠异位症血管生成及应激水平的影响[J].湖南中医药大学学报,2022,42(8):1266-1270[点击复制] |
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| 丹白颗粒对子宫内膜异位症大鼠异位症血管生成及应激水平的影响 |
| 姜艳玲,王金华,李发余,徐善荣,郭海龙 |
| (中药制药共性技术国家重点实验室, 山东 临沂 276006;福建医科大学附属第一医院妇科, 福建 福州 350005;日照市人民医院妇科, 山东 日照 276826) |
| 摘要: |
| 目的 研究丹白颗粒对子宫内膜异位症大鼠氧化应激和异位症血管生成的作用及机制。方法 选取子宫内膜异位症大鼠模型分为模型组、阳性药组及低、中、高剂量组，每组8只，另取8只大鼠设为假手术组。丹白颗粒低、中、高剂量组分别灌胃不同浓度丹白颗粒溶液连续给药2周，每天3次；假手术组和模型组给予等体积的蒸馏水。检测各组大鼠异位子宫内膜生长情况，检测血清血管内皮生长因子（vascular endothelial growth factor,VEGF）含量、微血管密度（microvessel density,MVD），测定促卵泡生成素（follicle stimulating hormone,FSH）、促黄体生成素（luteinizing hormone,LH）、雌二醇（estradiol,E2）、孕酮（proges-terone,P）水平，测定异位子宫内膜组织中的超氧化物歧化酶（superoxide dismutase,SOD）、丙二醛（malondialdehyde,MDA）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）水平，并进行比较分析。结果 与模型组相比，低、中、高剂量组大鼠的异位组织体积、异位组织重量、在位内膜厚度明显降低（P＜0.05），且降低幅度由大到小依次为高剂量组、中剂量组、低剂量组（P＜0.05）；低、中、高剂量组的VEGF、MVD、FSH、LH、E2、MDA水平低于模型组，P、SOD、GSH-Px水平高于模型组（P＜0.05）。结论 丹白颗粒可以有效控制大鼠子宫内膜异位症的发展，其机制可能与抑制异位症血管生成及降低氧化应激有关。 |
| 关键词: 丹白颗粒 大鼠 子宫内膜异位症 氧化应激 异位症血管生成 机制 |
| DOI：10.3969/j.issn.1674-070X.2022.08.007 |
| 投稿时间：2022-01-21 |
| 基金项目:国家重点研发计划（2019YFC1711200）。 |
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| Effect of Danbai Granules on heterotopic angiogenesis and stress levels of endometriosis rats |
| JIANG Yanling,WANG Jinhua,LI Fayu,XU Shanrong,GUO Hailong |
| (State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Linyi, Shandong, 276006, China;Department of Gynecology, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, China;Department of Gynecology, Rizhao City People's Hospital, Rizhao, Shandong 276826, China) |
| Abstract: |
| Objective To study the effect and mechanism of Danbai Granules on oxidative stress and heterotopic angiogenesis in endometriosis rats. Methods The endometriosis rat models were selected and divided into model group, positive drug group, and Danbai Granules low-dose, medium-dose and high-dose groups, with 8 rats in each group, and 8 rats were selected as sham operation group. Danbai Granules low-dose, medium-dose and high-dose groups were given different concentrations of Danbai Granules solution by gavage respectively for 2 weeks, 3 times a day, and the sham operation group and model group were given an equal volume of distilled water. The growth of ectopic endometrium, serum vascular endothelial growth factor (VEGF) and microvessel density (MVD) were detected. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and proges-terone (P) were measured. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in ectopic endometrial tissues were measured and compared. Results Compared with the model group, the volume of ectopic tissue, the weight of ectopic tissue and the thickness of eutopic intima in Danbai Granules low-dose, medium-dose and high-dose groups were significantly decreased (P<0.05), and the decreasing range from large to small was successively Danbai Granules high-dose, medium-dose, low-dose groups (P<0.05); the levels of VEGF, MVD, FSH, LH, E2 and MDA in the Danbai Granules low-dose, medium-dose and high-dose groups were lower than those in the model group, and the levels of P, SOD and GSH-Px were higher than those in the model group (P<0.05). Conclusion Danbai Granules can effectively control the development of endometriosis in rats, and its mechanism may be related to the inhibition of angiogenesis and the reduction of oxidative stress. |
| Key words: Danbai Granules rat endometriosis oxidative stress heterotopic angiogenesis mechanism |
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