| 引用本文: |
范洪桥,胡金辉,刘丽芳,吴雨薇.矾冰纳米乳对增生性瘢痕VEGF、Ang-1、TGF-β1与MMP-2表达的影响[J].湖南中医药大学学报,2022,42(3):361-366[点击复制] |
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| 矾冰纳米乳对增生性瘢痕VEGF、Ang-1、TGF-β1与MMP-2表达的影响 |
| 范洪桥,胡金辉,刘丽芳,吴雨薇 |
| (湖南中医药大学第一附属医院中医外科, 湖南 长沙 410007) |
| 摘要: |
| 目的 观察矾冰纳米乳对增生性瘢痕血管内皮生长因子（vascular endothelial growth factor, VEGF）、血管生成素-1（angiopoietin-1, Ang-1）、转化生长因子-β1（transforming growth factor-β1, TGF-β1）与基质金属蛋白酶-2（matrix metalloproteinase-2, MMP-2）表达的影响。方法 通过烧伤大鼠背部皮肤建立增生性瘢痕模型。144只SPF级SD大鼠随机分为空白对照组、模型组、曲安奈德组及低、中、高剂量组（矾冰纳米乳8.15、16.3、32.6 mg/mL），每组24只。连续给药35 d（除空白对照组外）。明显分别在第14、21、28、35天4个不同时间点采用空气栓塞法每组随机处死6只大鼠。采用HE染色法检测成纤维细胞数密度；采用Westernblot检测VEGF、Ang-1、TGF-β1、MMP-2蛋白表达。结果 与同时间点空白对照组比较，模型组成纤维细胞数密度及VEGF、Ang-1、TGF-β1、MMP-2蛋白表达均明显升高（P<0.05）。除14 d外，与同时间点模型组比较，曲安奈德组与低、中、高剂量组成纤维细胞数密度及VEGF、Ang-1、TGF-β1、MMP-2蛋白表达均明显降低（P<0.05）。与同时间点曲安奈德组比较，中剂量组成纤维细胞数密度及VEGF、Ang-1、TGF-β1、MMP-2蛋白表达均明显降低（P<0.05）；低、高剂量组成纤维细胞数密度及VEGF、Ang-1、TGF-β1、MMP-2蛋白表达均升高，差异无统计学意义（P>0.05）。模型组中，与前一时间点比较，成纤维细胞密度均明显升高（P<0.05）。曲安奈德组与低、中、高剂量组中，与前一时间点比较，成纤维细胞密度均明显降低（P<0.05）。空白对照组中，21 d与14 d比较，VEGF、Ang-1、TGF-β1蛋白表达均明显升高（P<0.05）；35 d与28 d比较，VEGF、Ang-1、TGF-β1蛋白表达均明显降低（P<0.05）。模型组中，28 d与21 d比较，TGF-β1蛋白表达明显升高（P<0.05）；35 d与28 d比较，VEGF、Ang-1蛋白表达均明显升高（P<0.05）。曲安奈德组中，28 d与21 d比较，TGF-β1蛋白表达明显降低（P<0.05）；35 d与28 d比较，TGF-β1、MMP-2蛋白表达均明显降低（P<0.05）。低剂量组中，35 d与28 d比较，TGF-β1、MMP-2蛋白表达均明显降低（P<0.05）。中剂量组中，28 d与21 d比较，TGF-β1蛋白表达明显降低（P<0.05）；35 d与28 d比较，VEGF、Ang-1蛋白表达均明显降低（P<0.05）。结论 矾冰纳米乳能降低增生性瘢痕模型大鼠成纤维细胞数密度及VEGF、Ang-1、TGF-β1、MMP-2蛋白表达，防治增生性瘢痕，以中剂量矾冰纳米乳疗效最佳。 |
| 关键词: 矾冰纳米乳 血管内皮生长因子 血管生成素-1 转化生长因子-β1 基质金属蛋白酶-2 增生性瘢痕 |
| DOI：10.3969/j.issn.1674-070X.2022.03.003 |
| 投稿时间：2021-07-31 |
| 基金项目:国家自然科学基金项目（81473680）；湖南省卫生健康委课题（20200667）；湖南省教育厅科学研究项目（19C1428）；湖南省高等学校"双一流"学科建设项目（湘教通[2018]469号）；湖南中医药大学科研计划项目（D2022108）；湖南中医药大学校级课题（2020XJJJ038）。 |
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| Effects of alum ice nanoemulsion on expression of VEGF, Ang-1, TGF-β1 and MMP-2 in hypertrophic scar |
| FAN Hongqiao,HU Jinhui,LIU Lifang,WU Yuwei |
| (Department of TCM Surgery, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To observe the effects of alum ice nanoemulsion on expression of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), transforming growth factor-β1 (TGF-β1) and matrix metalloproteinase-2 (MMP-2) in hypertrophic scar. Methods Hypertrophic scar model was established by burn rat dorsal skin. 144 SD rats (SPF grade) were divided into blank control group, model group, triamcinolone group, low-dose, medium-dose and high-dose group (alum ice nanoemulsion 8.15, 16.3, 32.6 mg/mL)， with 24 rats in each group. Continuous administration for 35 days (except for blank control group). Six rats in each group were randomly killed by air embolization at 4 different time points on day 14, 21, 28 and 35, respectively. The number density of fibroblasts was detected by HE staining. The protein expression levels of VEGF, ANG-1, TGF-β1 and MMP2 were detected by Western blot. Results Compared with blank control group at the same time point, the number density of fibroblasts and the protein expression of VEGF, Ang-1, TGF-β1 and MMP-2 were significantly increased in model group (P<0.05). Compared with model group at the same time point except the day 14, the number density of fibroblasts and the protein expression of VEGF, Ang-1, TGF-β1 and MMP-2 were decreased in triamcinolone group, low-dose, medium-dose and high-dose group (P<0.05). Compared with triamcinolone group, the number density of fibroblasts and the protein expression of VEGF, Ang-1, TGF-β1 and MMP-2 were decreased in low-dose group at the same time point; the number density of fibroblasts and the protein expression of VEGF, Ang-1, TGF-β1 and MMP-2 were significantly increased in low-dose and high-dose group, and there was no statistical significance (P>0.05). In model group, compared with the previous time point, the density of fibroblast was significantly increased (P<0.05). Compared with the previous time point, the density of fibroblasts in triamcinolone group, low, medium and high dose groups was significantly decreased (P<0.05). In blank control group, the protein expression levels of VEGF, ANG-1 and TGF-β1 were significantly increased on day 21 compared with day 14 (P<0.05); compared with day 28, the protein expression leves of VEGF and ANG-1 were significantly decreased on day 35 (P<0.05). In the model group, the expression of TGF-β1 protein was significantly increased on day 28 compared with day 21 (P<0.05); the protein expression levels of VEGF and ANG-1 were significantly increased on day 35 compared with day 28 (P<0.05). In triamcinolone group, the expression of TGF-β1 protein was significantly decreased on day 28 compared with day 21 (P<0.05); compared with day 28, the protein expression levels of TGF-β1 and MMP-2 were significantly decreased on day 35 (P<0.05). In the low-dose group, the protein expression levels of TGF-β1 and MMP-2 were significantly decreased on day 35 compared with day 28 (P<0.05). In the medium-dose group, the expression of TGF-β1 protein was significantly decreased on day 28 compared with day 21 (P<0.05); compared with day 28, VEGF and ANG-1 protein expression levels were significantly decreased on day 35 (P<0.05). Conclusion Alum ice nanoemulsion can reduce the number density of fibroblasts and the protein expression of VEGF, Ang-1, TGF-β1 and MMP-2 in hypertrophic scar model rats, and prevent hypertrophic scar formation, with the best effect in medium-dose alum ice nanoemulsion. |
| Key words: alum ice nanoemulsion vascular endothelial growth factor angiopoietin-1 transforming growth factor-β1 matrix metalloproteinase-2 hypertrophic scar |
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