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刘豪杰,陈雪蕾.芦荟大黄素对胃癌SGC-7901细胞Cav-1/PTEN/PI3K/PKB信号通路及侵袭、转移的影响[J].湖南中医药大学学报,2021,41(6):827-831[点击复制] |
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| 芦荟大黄素对胃癌SGC-7901细胞Cav-1/PTEN/PI3K/PKB信号通路及侵袭、转移的影响 |
| 刘豪杰,陈雪蕾 |
| (鄂州市中心医院消化科, 湖北 鄂州 436000) |
| 摘要: |
| 目的 探讨芦荟大黄素对胃癌SGC-7901细胞Cav-1/PTEN/PI3K/PKB信号通路及侵袭、转移的影响。方法 将复苏后胃癌SGC-7901细胞分为空白组、对照组和实验组，空白组给予灭活胎牛血清的MEM培养基正常培养，对照组及实验组在空白组基础上分别加入姜黄素40 μmol/L和芦荟大黄素50 μmol/L培养，3组细胞均培养24 h。采用划痕实验检测细胞迁移闭合率，采用Transwell小室检测侵袭细胞个数，采用Western blot检测上皮间质化（EMT）相关蛋白E钙黏蛋白（E-cad）、N钙黏蛋白（N-cad）、波形蛋白（Vim）以及基质金属蛋白-9（MMP-9）、小窝蛋白-1（Cav-1）、PTEN磷脂酰肌醇-3激酶（PI3K）、蛋白激酶B（PKB）信号通路的表达。结果 与空白组比较，对照组及实验组细胞划痕闭合率及侵袭细胞数均降低（P<0.05），Vim、N-cad、PI3K、PKB、MMP-9蛋白表达降低（P<0.05），E-cad、Cav-1、PTEN蛋白表达升高（P<0.05）；与对照组比较，实验组细胞划痕闭合率、侵袭细胞数及上述蛋白表达均无明显差异（P>0.05）。结论 芦荟大黄素可抑制胃癌SGC-7901细胞迁移及侵袭能力，其机制可能与抑制EMT及改善Cav-1/PTEN/PI3K/PKB信号通路表达有关。 |
| 关键词: 芦荟大黄素 胃癌细胞 SGC-7901 小窝蛋白-1 PTEN磷脂酰肌醇-3激酶 蛋白激酶B 迁移 侵袭 |
| DOI：10.3969/j.issn.1674-070X.2021.06.002 |
| 投稿时间：2021-03-14 |
| 基金项目:湖北省卫计委科研课题项目（Z2016668）。 |
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| Effects of Aloe Emodin on Cav-1/PTEN/PI3K/PKB Signaling Pathway, Invasion and Metastasis of Gastric Cancer SGC-7901 Cells |
| LIU Haojie,CHEN Xuelei |
| (Department of Gastroenterology, Ezhou Central Hospital, Ezhou, Hubei 436000, China) |
| Abstract: |
| Objective To investigate the effects of aloe emodin on Cav-1/PTEN/PI3K/PKB signaling pathway, invasion and metastasis of gastric cancer SGC-7901 cells. Methods After resuscitation, SGC-7901 cells were divided into blank group, control group and experimental group, the blank group was treated with MEM medium containing inactivated fetal bovine serum, on the basis of the blank group, the control group and experimental group were cultured with curcumin 40 μmol/L and aloe emodin 50 μmol/L, respectively. The three groups of cells were cultured for 24 hours. The migration and closure rate of cells was detected by scratch test, the number of invasive cells was detected by Transwell chamber, and the expression levels of epithelial mesenchymal transition (EMT) related proteins E-cadherin (E-cad), N-cadherin (N-cad), vimentin (Vim), matrix metalloproteinase-9 (MMP-9), caveolin-1 (Cav-1), PTEN phosphatidylinositol-3 kinase (PI3K) and protein kinase B (PKB) signaling pathways were detected by Western blot. Results Compared with the blank group, the wound closure rate and the number of invasive cells in the control group and experimental group were decreased (P<0.05), and the expression levels of Vim, N-cad, PI3K, PKB and MMP-9 protein were decreased (P<0.05), while the expression levels of E-cad, Cav-1 and PTEN protein were increased (P<0.05). Compared with the control group,there were no significant differences in wound closure rate, number of invasive cells and expression of the above proteins in the experimental group (P>0.05). Conclusion Aloe emodin can inhibit the migration and invasion of gastric cancer SGC-7901 cells, and its mechanism may be related to the inhibition of EMT and the improvement of Cav-1/PTEN/PI3K/PKB signal pathway expression. |
| Key words: aloe emodin gastric cancer cell SGC-7901 caveolin-1 PTEN phosphatidylinositol-3 kinase protein kinase B migration invasion |
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