| 引用本文: |
熊雨,刘洁,邹攀,苏芮,钟文良,江志超,王贤文,何迎春.益气解毒方中苯丙素类化合物对鼻咽癌细胞增殖效应的影响[J].湖南中医药大学学报,2021,41(5):701-706[点击复制] |
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| 益气解毒方中苯丙素类化合物对鼻咽癌细胞增殖效应的影响 |
| 熊雨,刘洁,邹攀,苏芮,钟文良,江志超,王贤文,何迎春 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省脑科医院, 湖南 长沙 410005;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心, 湖南 长沙 410208;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208;湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心, 湖南 长沙 410208;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨益气解毒方中苯丙素类化合物异欧前胡素、阿魏酸、绿原酸对人鼻咽癌CNE2细胞增殖效应的抑制作用及机制研究。方法 使用实时无标记细胞功能分析技术（RTCA）动态监测不同浓度的3种苯丙素类化合物对离体人鼻咽癌CNE2细胞生长曲线的影响；CytationTM 5高通量活细胞成像分析检测系统分别监测3种不同浓度苯丙素类化合物对人鼻咽癌CNE2细胞形态改变；Western blot法检测3种苯丙素类化合物对人鼻咽癌CNE2细胞增殖相关蛋白增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）、X连锁的凋亡蛋白抑制剂（X-linked inhibitor of apoptosis protein，XIAP）、Survivin表达的影响。结果 RTCA实时监测结果显示异欧前胡素、阿魏酸、绿原酸均能不同程度地抑制CNE2细胞增殖，存在药物浓度越高抑制效应越明显的趋势。CytationTM 5检测结果显示，与对照组相比，3种苯丙素类化合物作用36 h后，大量CNE2细胞出现细胞漂浮死亡，细胞膜破溃、细胞结构模糊等形态变化。Western blot检测结果显示，与对照组相比，异欧前胡素、阿魏酸、绿原酸能明显抑制CNE2细胞中增殖相关蛋白PCNA、XIAP、Survivin的表达（P<0.05）。结论 益气解毒方中主要苯丙素类化合物异欧前胡素、阿魏酸、绿原酸均可以不同程度抑制人鼻咽癌CNE2细胞增殖，其机制可能与下调增殖相关蛋白PCNA、XIAP、Survivin的表达有关。 |
| 关键词: 鼻咽癌 益气解毒方 苯丙素类化合物 细胞增殖 异欧前胡素 阿魏酸 绿原酸 |
| DOI：10.3969/j.issn.1674-070X.2021.05.009 |
| 投稿时间：2021-01-21 |
| 基金项目:国家自然科学基金项目（81973914，81874408）；湖南省自然科学基金项目（2019JJ40216，2018JJ2219）。 |
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| Effects of the Phenylpropanoids in Yiqi Jiedu Formula on the Proliferation of Nasopharyngeal Carcinoma Cells |
| XIONG Yu,LIU Jie,ZOU Pan,SU Rui,ZHONG Wenliang,JIANG Zhichao,WANG Xianwen,HE Yingchun |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Brain Hospital of Hunan Province, Changsha, Hunan 410005, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the inhibitory effect and mechanism of the phenylpropanoid compounds isoimperatorin, ferulic acid and chlorogenic acid in Yiqi Jiedu Formula on the proliferation of human nasopharyngeal carcinoma CNE2 cells. Methods Real-time unlabeled cell function analysis technique (RTCA) was used to dynamically monitor the effects of three phenylpropanoids at different concentrations on the proliferation of human nasopharyngeal carcinoma CNE2 cells. CytationTM 5 high-throughput live cell imaging analysis system was used to monitor the effects of three phenylpropanoids at different concentrations on the morphological changes of human nasopharyngeal carcinoma CNE2 cells. Western blot method was used to detect the effects of three phenylpropanoids on human nasopharyngeal carcinoma CNE2 cells proliferating cell nuclear antigen (PCNA), X-linked inhibitor of apoptosis protein (XIAP), and Survivin expression. Results RTCA results showed that isoperatorin, ferulic acid, and chlorogenic acid can inhibit the proliferation of CNE2 cells to varying degrees, and there was a trend that the higher the drug concentration, the more obvious the inhibitory effect. The CytationTM 5 results showed that compared with the control group, a large number of CNE2 cells had cell floating death, cell membrane rupture, cell structure fuzzy and other morphological changes after the three phenylpropanoids were treated for 36 hours. Western blot detection results showed that compared with the control group, isoperatorin, ferulic acid, and chlorogenic acid can significantly inhibit the expression of proliferation-related proteins PCNA, XIAP, and Survivin in CNE2 cells (P<0.05). Conclusion The main phenylpropanoid compounds, isoimperatorin, ferulic acid and chlorogenic acid in Yiqi Jiedu Formula, which can inhibit the proliferation of human nasopharyngeal carcinoma CNE2 cells to varying degrees. The mechanism may be related to the down-regulation of proliferation-related proteins PCNA, XIAP, and Survivin expression level. |
| Key words: nasopharyngeal carcinoma Yiqi Jiedu Formula phenylpropanoid compounds cell proliferation isoimperatorin ferulic acid chlorogenic acid |
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