| 引用本文: |
江玉婷,李铁浪,李江山,蒋全睿,匡小霞,袁媛,危威,朱晓晴,吴琼.推拿按法对慢性激痛点模型大鼠骨骼肌超微结构的影响[J].湖南中医药大学学报,2021,41(1):85-90[点击复制] |
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| 推拿按法对慢性激痛点模型大鼠骨骼肌超微结构的影响 |
| 江玉婷,李铁浪,李江山,蒋全睿,匡小霞,袁媛,危威,朱晓晴,吴琼 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察推拿按法对慢性激痛点模型大鼠骨骼肌超微结构的影响，探讨推拿按法对慢性激痛点的治疗作用。方法 将健康雄性SD大鼠随机分为空白组、模型组、按法组、利多卡因组，每组6只。采用钝性打击结合离心运动方式建立慢性激痛点大鼠模型。按法组大鼠予按法刺激局部激痛点，隔天治疗1次，每次7.5 min，共治疗7次；利多卡因组大鼠予0.5 mL的1%利多卡因注射液治疗，6 d 1次，共治疗3次。2周干预结束后，采用HE染色观察各组大鼠骨骼肌显微结构变化；采用透射电镜观察各组大鼠骨骼肌组织超微结构变化。结果（1）光镜下，模型组可见异常梭形形态肌纤维，表现为中间膨大，两端变窄，伴大量炎症细胞浸润和核内移现象；与模型组比较，按法组和利多卡因组均可见肌纤维粗细均匀、排列较整齐，炎症细胞减少。（2）电镜下，模型组肌原纤维排列紊乱，大量肌纤维断裂、破损，线粒体数量减少，结构异常，出现肿胀变圆、嵴结构减少或呈空泡状；按法组和利多卡因组表现接近，与模型组比较，肌原纤维排列趋于整齐，线粒体数量增多，结构恢复正常，呈细长杆状和卵圆形，或出现融合形态。各组肌节长度测量结果显示，模型组与空白组比较，肌节长度明显缩短，差异有显著统计学意义（P<0.01）；模型组与按法组、利多卡因组比较，差异有统计学意义（P<0.05）。结论 慢性激痛点病理特征为骨骼肌肌节挛缩、线粒体结构和数量受损。按法治疗能够舒张挛缩的肌节、促进线粒体的损伤修复，起到对慢性激痛点的去活化作用。 |
| 关键词: 慢性激痛点 推拿按法 骨骼肌 超微结构 肌纤维 |
| DOI：10.3969/j.issn.1674-070X.2021.01.015 |
| 投稿时间：2020-08-28 |
| 基金项目:国家自然科学基金面上项目（81973975）；湖南中医药大学第一附属医院湖南省院士专家工作站（石学敏）开放基金项目（2019YSZJJ01）；湖南中医药大学开放性基金项目（2018ZYX07）；湖南省研究生科研创新项目（CX20190559）。 |
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| Effect of Pressing Manipulation in Massage on Ultrastructure of Skeletal Muscle in Rats with Chronic Myofascial Trigger Points |
| JIANG Yuting,LI Tielang,LI Jiangshan,JIANG Quanrui,KUANG Xiaoxia,YUAN Yuan,WEI Wei,ZHU Xiaoqing,WU Qiong |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of pressing manipulation in massage on the ultrastructure of skeletal muscle with chronic myofascial trigger points (MTrPs) in rat model, so as to explore the therapeutic effect of pressing manipulation in massage on chronic MTrPs. Methods Healthy male SD rats were randomly divided into blank group, model group, pressing manipulation group and lidocaine group, with 6 rats in each group. Rat model of chronic MTrPs was established by blunt impact combined with eccentric exercise. The rats in the pressing manipulation group were stimulated by the local MTrPs, once every other day, 7.5 minutes each time, 7 times in total; the rats in the lidocaine group were treated with 0.5 mL 1% lidocaine injection once every 6 days for 3 times. After 2 weeks of intervention, HE staining was used to observe the changes of skeletal muscle microstructure, and transmission electron microscope was used to observe the changes of skeletal muscle ultrastructure. Results (1) Under light microscope, abnormal fusiform muscle fibers were observed in the model group, which showed enlargement in the middle and narrowing at both ends, accompanied by a large number of inflammatory cells infiltration and nuclear internalization. Compared with the model group, the muscle fibers of the pressing manipulation group and the lidocaine group were more uniform, more orderly, and less inflammatory cells. (2) Under electron microscope, myofibrils in the model group were disordered, a large number of myofibrils were broken and damaged, the number of mitochondria was reduced, and the structure was abnormal, such as swelling, rounding, crest reduction or vacuolation. The results of the pressing manipulation group and the lidocaine group were similar. Compared with the model group, myofibril arrangement tended to be more orderly, the number of mitochondria increased, the structure returned to normal, and the myofibril was elongated, rod-shaped and ovo-shaped, or there were fusion forms. The results of myosome length measurement in each group showed that compared with the blank group, the sarcomere length of the model group was significantly shortened, and the results were significantly different (P<0.01). There were significant differences between the model group, the pressing manipulation group and the lidocaine group (P<0.05). Conclusion The pathological features of MTrPs are musculoskeletal muscle contracture and damaged mitochondrial structure and quantity. Pressing manipulation therapy can relax the contracture of sarcomere, promote the damage repair of mitochondria, and inactivating of the chronic MTrPs. |
| Key words: chronic myofascial trigger points pressing manipulation in massage skeletal muscle ultrastructure muscle fiber |
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