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罗宗婷,庞书勤,周建,陈芳,洪雪珮,陈锦秀,葛莉,戴燕铃,李霞,王雅立.薯蓣粥对2型糖尿病大鼠肝脏糖异生的影响[J].湖南中医药大学学报,2021,41(1):46-52[点击复制] |
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| 薯蓣粥对2型糖尿病大鼠肝脏糖异生的影响 |
| 罗宗婷,庞书勤,周建,陈芳,洪雪珮,陈锦秀,葛莉,戴燕铃,李霞,王雅立 |
| (福建中医药大学护理学院, 福建 福州 350122) |
| 摘要: |
| 目的 观察薯蓣粥对2型糖尿病（type 2 diabetes mellitus，T2DM）大鼠磷酸烯醇式丙酮酸羧激酶（phosphoenolpyruvate carboxykinase，PEPCK）、葡萄糖-6-磷酸酶（glucose-6-phosphatase，G6Pase）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-6（interleukin-6，IL-6）、C-反应蛋白（C-reactive protein，CRP）和脂联素（adiponectin，APN）等与肝脏糖异生相关指标的变化，从肝脏糖异生角度探讨薯蓣粥的降糖机制，为薯蓣粥的临床应用提供科学依据。方法 60只Wistar大鼠随机分为空白组10只和造模组50只。造模组采用高糖高脂饲料联合腹腔注射小剂量链脲佐菌素（streptozotocin，STZ）制备T2DM大鼠模型，将造模成功的40只大鼠随机分为模型组、薯蓣粥组、二甲双胍组和联合治疗组，各组10只。每组给予相应药物连续灌胃给药6周后，全自动生化分析仪检测空腹血糖（fasting blood glucose，FBG）；ELISA检测血清TNF-α、IL-6、CRP和APN含量；RT-qPCR检测PEPCK和G6Pase基因（mRNA）表达；免疫组化检测PEPCK和G6Pase蛋白表达。结果 与空白组比较，模型组大鼠FBG显著升高（P<0.01）；血清TNF-α、IL-6、CRP水平上升，APN水平下降（P<0.01）；肝组织PEPCK、G6Pase mRNA及蛋白表达均增加（P<0.01）。与模型组比较，薯蓣粥组、二甲双胍组和联合治疗组FBG显著降低（P<0.01）；血清TNF-α、IL-6、CRP水平下降，APN水平上升（P<0.05）；肝组织PEPCK、G6Pase mRNA及蛋白表达均减少（P<0.01）；且联合治疗组较薯蓣粥组应用效果更优（P<0.05）。结论 薯蓣粥能够降低T2DM大鼠FBG水平，其作用机制可能与下调T2DM大鼠血清TNF-α、IL-6、CRP水平和上调APN水平，进而减少肝组织PEPCK、G6Pase mRNA及蛋白的表达，减弱肝脏糖异生有关。 |
| 关键词: 2型糖尿病 肝脏糖异生 薯蓣粥 空腹血糖 |
| DOI：10.3969/j.issn.1674-070X.2021.01.009 |
| 投稿时间：2020-06-02 |
| 基金项目:国家自然科学基金项目（81904269）；福建省自然科学基金项目（2017J01833）；福建中医药大学2019年校管课题护理学科开放课题（X2019040-学科）。 |
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| Effects of Yam Gruel on Hepatic Gluconeogenesis of Type 2 Diabetic Rats |
| LUO Zongting,PANG Shuqin,ZHOU Jian,CHEN Fang,HONG Xuepei,CHEN Jinxiu,GE Li,DAI Yanling,LI Xia,WANG Yali |
| (School of Nursing, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China) |
| Abstract: |
| Objective To observe the effects of yam gruel on phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G6Pase), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP), adiponectin (APN) and other indicators related to hepatic gluconeogenesis in rats with type 2 diabetes(T2DM), and to explore the hypoglycemic mechanism of yam gruel from the perspective of hepatic gluconeogenesis, so as to provide a scientific basis for the clinical application of yam gruel. Methods 60 Wistar rats were randomly divided into the control group (n=10) and model groups (n=50). Rats in model groups were fed with high-sugar/high-fat diet, and combined with an intraperitoneal injection of low-dose streptozotocin (STZ) to induce T2DM rats model. T2DM rats were selected and randomly divided into the model group, the yam gruel group, the metformin group, and the combined treatment group. Ten rats in each group. After 6 weeks of continuous gavage of the corresponding drug in each group, an automatic biochemical analyzer was used to detect fasting blood glucose (FBG) levels. ELISA kits were used to detect the concentrations of serum TNF-α, IL-6, CRP and APN. The mRNA expressions of PEPCK and G6Pase were detected by RT-qPCR. The protein expressions of PEPCK and G6Pase were detected by immunohistochemical. Results Compared with the control group, the FBG levels in the model group were significantly increased (P<0.01), the serum levels of TNF-α, IL-6 and CRP were increased while the serum levels of APN were decreased in the model group (P<0.01), both mRNA and protein levels of PEPCK and G6Pase in the liver tissues were significantly elevated in the model group (P<0.01). Compared with the model group, the FBG levels were significantly decreased in the yam gruel group, the metformin group and the combined treatment group (P<0.01), the serum levels of TNF-α, IL-6 and CRP were decreased, while the serum levels of APN were increased in the yam gruel group, the metformin group and the combined treatment group (P<0.05), both mRNA and protein levels of PEPCK and G6Pase in the liver tissues were significantly decreased in the yam gruel group, the metformin group and the combined treatment group (P<0.01). And the combined treatment group showed better efficacy than the yam gruel group (P<0.01). Conclusion Yam gruel has an effect on lowering FBG in T2DM rats, and its mechanism may be related to the down-regulation of serum TNF-α, IL-6, CRP, and the up-regulation of APN levels in T2DM rats, and then reducing the expressions of mRNA and protein levels of PEPCK and G6pase to inhibit excessive hepatic gluconeogenesis. |
| Key words: type 2 diabetes hepatic gluconeogenesis yam gruel fasting blood glucose |
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