| 引用本文: |
彭俊,蒋鹏飞,王英,刘家琪,潘坤,周亚莎,徐剑,彭清华.枸杞子加丹参对RCS（rdy-/-,p-/-）大鼠视网膜组织形态及感光细胞凋亡的影响[J].湖南中医药大学学报,2020,40(9):1086-1089[点击复制] |
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| 枸杞子加丹参对RCS（rdy-/-,p-/-）大鼠视网膜组织形态及感光细胞凋亡的影响 |
| 彭俊,蒋鹏飞,王英,刘家琪,潘坤,周亚莎,徐剑,彭清华 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察枸杞子加丹参对皇家外科学院（royal college surgeons，RCS）（rdy-/，p-/-）大鼠视网膜组织形态及感光细胞凋亡的影响。方法 40只RCS大鼠随机平均分为空白组、模型组、枸杞组、丹参组、杞参组，空白组及模型组灌胃蒸馏水，枸杞组灌胃枸杞子中药配方颗粒水溶液，丹参组灌胃丹参中药配方颗粒水溶液，杞参组灌胃枸杞子加丹参中药配方颗粒水溶液。灌胃28 d后，HE染色观察各组RCS大鼠视网膜组织形态，TUNEL法检测视网膜感光细胞凋亡情况，并计算细胞凋亡指数（apoptosis index，AI）。结果 HE染色显示：空白组大鼠视网膜各层结构排列有序，形态规则；模型组大鼠视网膜各层结构紊乱，视网膜变薄；枸杞组与丹参组视网膜各层组织结构同模型组大鼠结构类似；杞参组视网膜外层萎缩现象较模型组减轻。TUNEL染色显示：空白组大鼠视网膜未见明显感光细胞凋亡；模型组大鼠视网膜各层见大量感光细胞凋亡；枸杞组与丹参组大鼠视网膜见大量感光细胞凋亡；杞参组大鼠视网膜见少量感光细胞凋亡。视网膜感光细胞AI：杞参组（14.13±5.64）%明显低于模型组（24.38±9.62）%、枸杞组（21.25±10.21）%、丹参组（19.25±7.98）%，差异有统计学意义（P<0.05）。结论 枸杞子加丹参可以减轻RCS大鼠视网膜感光细胞凋亡，从而保护视功能。 |
| 关键词: 视网膜色素变性 RCS大鼠 细胞凋亡 枸杞子 丹参 |
| DOI：10.3969/j.issn.1674-070X.2020.09.008 |
| 投稿时间：2019-01-20 |
| 基金项目:国家自然科学基金项目（81804150）；湖南省自然科学基金面上项目（2019JJ40226）；湖南省教育厅创新平台开放基金项目（16K065）；湖南中医药大学中医诊断学国家重点学科开放基金优先资助项目（2015ZYZD02）；中医药防治五官科疾病湖南省重点实验室建设项目（2017TP1018）；国家中医药管理局中医眼科学重点学科建设项目（ZK1801YK015）；湖南省中医五官科学重点学科建设项目。 |
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| Effects of Fructus Lycii and Radix Salviae Miltiorrhizae on Retinal Tissue Morphology and Phatoreceptor Apoptosis of RCS (rdy-/, p-/-) Rats |
| PENG Jun,JIANG Pengfei,WANG Ying,LIU Jiaqi,PAN Kun,ZHOU Yasha,XU Jian,PENG Qinghua |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases in Hunan Province, Changsha, Hunan 410208, China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases in Hunan Province, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases in Hunan Province, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of Fructus Lycii and Radix Salviae Miltiorrhizae on retinal tissue morphology and photoreceptor cell apoptosis in royal college surgeons (RCS) (rdy-/, p-/-) rats. Methods A total of 40 RCS rats were randomly and equally divided into a blank group, a model group, a Fructus Lycii group, a Radix Salviae Miltiorrhizae group, and a Fructus Lycii and Radix Salviae Miltiorrhizae group. The blank group and the model group were given distilled water by gavage. The Fructus Lycii group was given Fructus Lycii Chinese medicine formula granule water solution by gavage. The Radix Salviae Miltiorrhizae group was given Radix Salviae Miltiorrhizae Chinese medicine formula granule water solution by gavage. The Fructus Lycii and Radix Salviae Miltiorrhizae group was given Fructus Lycii and Radix Salviae Miltiorrhizae Chinese medicine formula granule water solution by gavage. In 28 days after gavage, the retinal tissue morphology of RCS rats in each group was observed by HE staining. Apoptosis of retinal photoreceptor cells was detected by TUNEL method, and apoptosis index (AI) was calculated. Results HE staining showed that the layers of the retina in the blank group were arranged in an orderly and regular morphology; the structures of the layers of the retina in the model group were disordered and the retina was thin; the structure of each layer of retina in the Fructus Lycii group and the Radix Salviae Miltiorrhizae group was similar to that of the model group; outer retinal atrophy in the Fructus Lycii and Radix Salviae Miltiorrhizae group was less than that in model group. TUNEL staining showed that there were no obvious phatoreceptor apoptotic cells in the retina of the blank group; a large number of phatoreceptor apoptotic cells were seen in each layer of the retina of the model group; a large number of phatoreceptor apoptotic cells were seen in the retina of the Fructus Lycii group and the Radix Salviae Miltiorrhizae group; few phatoreceptor apoptotic cells were seen in the retina of rats in the Fructus Lycii and Radix Salviae Miltiorrhizae group. For retinal photoreceptor cell AI comparison, (14.13±5.64)% of the Fructus Lycii group and the Radix Salviae Miltiorrhizae group was significantly lower than the model group (24.38±9.62)%, the Fructus Lycii group (21.25±10.21)% and the Radix Salviae Miltiorrhizae group (19.25±7.98)%, and the difference was statistically significant (P<0.05). Conclusion Fructus Lycii and Radix Salviae Miltiorrhizae can alleviate the apoptosis of retinal phatoreceptor cells of RCS rats, thus protecting visual function. |
| Key words: retinitis pigmentosa RCS rats apoptosis Fructus Lycii Radix Salviae Miltiorrhizae |
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