| 引用本文: |
黄强,汪亚楠,韩飞,刘明.基于网络药理学的桂枝甘草汤抗失眠作用的潜在机制研究[J].湖南中医药大学学报,2020,40(4):452-459[点击复制] |
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| 基于网络药理学的桂枝甘草汤抗失眠作用的潜在机制研究 |
| 黄强,汪亚楠,韩飞,刘明 |
| (国家知识产权局专利局审查协作江苏中心, 江苏 苏州 215163;沈阳药科大学, 辽宁 沈阳 110016) |
| 摘要: |
| 目的 利用网络药理学技术对桂枝甘草汤抗失眠的潜在作用机制进行探讨研究。方法 首先，利用TCMSP、Swiss、SuperPred和Stitch数据库寻找与桂枝甘草汤方中2味中药材有关的化学成分及其可能的作用靶点，并设定OB ≥ 30%、DL ≥ 0.18为阈值对化学成分进行筛选，再利用文献检索加以补充；同时，通过OMIM、TTD、Gene Card、Pharm Gkb等数据库获取与失眠症相关的靶标，并进一步建立桂枝甘草汤抗失眠作用的靶点；然后，利用DAVID数据库对失眠症相关的作用靶点进行GO及KEGG富集分析；最后，采用Cytoscape软件构建"单味药-活性成分-作用靶点"相互作用网络并进行度值和介数值分析，确认关键靶点和关键通路。结果 在桂枝甘草汤中，共筛选出101个与失眠相关的潜在活性成分，主要涉及MAPT、ESR1、CYP19A1等85个潜在作用靶点；GO生物功能分析共包含293条富集结果，主要涉及蛋白结合、质膜等；KEGG富集分析得到18条代谢通路，主要包括神经活性配体-受体相互作用通路、钙信号通路、5-羟色胺能突触通路等。结论 本研究揭示了桂枝甘草汤治疗失眠症的"多成分-多靶点-多途径"的作用特点，并预测了其可能的活性成分、关键靶点和作用通路，为其药效物质基础和作用机制研究提供了理论基础。 |
| 关键词: 失眠 桂枝甘草汤 网络药理学 靶点 作用机制 |
| DOI：10.3969/j.issn.1674-070X.2020.04.013 |
| 投稿时间：2019-08-28 |
| 基金项目: |
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| Potential Mechanism of Anti-insomnia Effect of Guizhi Gancao Decoction Based on Network Pharmacology |
| HUANG Qiang,WANG Yanan,HAN Fei,LIU Ming |
| (Patent Examination Cooperation Jiangsu Center of the Patent Office, State Intellectual Property Office, Suzhou, Jiangsu 215163, China;Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China) |
| Abstract: |
| Objective To study the potential mechanism of the anti-insomnia effect of Guizhi Gancao Decoction (GGD) by using network pharmacological technology. Methods Firstly, TCMSP, Swiss, SuperPred and Stitch databases were used to search for the chemical compounds and the possible targets related to the 2 herbs in GGD. OB ≥ 30%, DL ≥ 0.18 were set as screening conditions to screen for chemical compounds. Literatures were searched for supplement. Meanwhile, the targets related to insomnia were screened through OMIM, TTD, Gene Card and Pharm Gkb databases. Moreover, the interactive targets of GGD and insomnia were further constructed. Then, GO analysis and KEGG enrichment analysis of insomnia related targets were performed using DAVID database. Finally, degree and betweenness analysis were performed to find key targets and key pathways after the "single herb-active compounds-acting targets" interaction network was constructed by Cytoscape software. Results A total of 101 potential active compounds related to insomnia were screened in GGD, which mainly involved 85 potential targets such as MAPT, ESR1 and CYP19A1. A total of 293 enrichment results were obtained by GO analysis, mainly involving protein binding, plasma membrane, etc. 18 metabolic pathways were obtained through KEGG analysis, mainly including neuroactive ligand-receptor interaction pathway, calcium signaling pathway, serotonergic synapse and etc. Conclusion This study revealed the action characteristics of GGD with "multi-compounds, multi-targets and multi-pathways" in the treatment of insomnia and predicted its possible active ingredients, key targets and action pathways, which provided a theoretical basis for the study of its pharmacodynamic material basis and action mechanism. |
| Key words: insomnia Guizhi Gancao Decoction network pharmacology targets action mechanism |
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