| 引用本文: |
曹惠敏,杨云霜,余雷,杜磊,李延晖,张蓉.补肾解毒方对小鼠胸腺、脾脏及外周血白细胞、淋巴细胞的辐射防护作用[J].湖南中医药大学学报,2020,40(3):280-285[点击复制] |
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| 补肾解毒方对小鼠胸腺、脾脏及外周血白细胞、淋巴细胞的辐射防护作用 |
| 曹惠敏,杨云霜,余雷,杜磊,李延晖,张蓉 |
| (苏州大学, 215000;北京市隆福医院, 北京 100010;火箭军特色医学中心, 北京 100088) |
| 摘要: |
| 目的 探讨自拟中药补肾解毒方对小鼠胸腺、脾脏、白细胞及淋巴细胞的影响。方法 将90只清洁级小鼠随机分为空白对照组、辐射模型组、氨磷汀阳性对照组、补肾解毒方预防组、补肾解毒方防治结合组。造模前各组分别进行相应处理。除空白对照组外，其余4组小鼠均接受剂量为6 Gy的60Co γ射线的辐射；并于辐射后第1天、第7天、第14天分批处死小鼠，记录小鼠体质量及胸腺、脾脏质量，计算脾脏指数和胸腺指数；眼球采血并采用全自动生化仪测定外周血白细胞、淋巴细胞总数。结果 （1）辐射后第1天，与空白对照组相比，各组小鼠胸腺指数、脾脏指数均明显下降（P<0.01）；辐射后第7天，补肾解毒方预防组和补肾解毒方防治结合组胸腺指数明显高于辐射模型组（P<0.05或P<0.01）；辐射后第14天，补肾解毒方预防组、补肾解毒方防治结合组胸腺指数、脾脏指数高于氨磷汀阳性对照组和辐射模型组（P<0.01或P<0.05）。（2）辐射后第1天，与空白对照组相比，各组小鼠白细胞、淋巴细胞均明显下降（P<0.01）；辐射后第7天，补肾解毒方预防组和补肾解毒方防治结合组淋巴细胞数量明显高于氨磷汀阳性对照组（P<0.05）；辐射后第14天，补肾解毒方预防组、补肾解毒方防治结合组小鼠白细胞、淋巴细胞明显高于辐射模型组和氨磷汀阳性对照组（P<0.01或P<0.05）。结论 补肾解毒方能够促进辐射后胸腺和脾脏的恢复，提高外周血白细胞和淋巴细胞总数，具有良好的辐射防护作用。 |
| 关键词: 补肾解毒方 辐射 免疫器官 免疫细胞 |
| DOI：10.3969/j.issn.1674-070X.2020.03.007 |
| 投稿时间：2019-05-13 |
| 基金项目:国家自然基金面上项目（81673873，81703979）。 |
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| Radiation Protection Effect of Bushen Jiedu Prescription on Mice Thymus, Spleen, White Blood Cells and Lymphocytes |
| CAO Huimin,YANG Yunshuang,YU Lei,DU Lei,LI Yanhui,ZHANG Rong |
| (Soochow University, Suzhou, Jiangsu 215000, China;Beijing Longfu Hospital, Beijing 100010, China;Rocket Army Special Medical Center, Beijing 100088, China) |
| Abstract: |
| Objective To explore the effect of self-made Chinese materia medica Bushen Jiedu Prescription on thymus, spleen, white blood cells, lymphocytes in mice. Methods A total of 90 clean-grade mice were randomly divided into a blank control group, a radiation model group, a amifostine-positive control group, a Bushen Jiedu Prescription prevention group, and a Bushen Jiedu Prescription prevention and treatment group. Each group was processed separately before modeling. Except for the blank control group, the other four groups received a dose of 6 Gy of 60Co γ ray radiation. The mice were killed in batches on the 1st, 7th, and 14th day after radiation. The body weight, thymus and spleen mass were recorded and the spleen coefficient and thymus coefficient were calculated. Blood was taken from the eyeball and blood routine test was performed using an automatic biochemical analyzer to count the total number of white blood cells and lymphocytes. Results (1) On the 1st day after radiation, compared with the blank control group, the thymus index and spleen index of the mice in each group decreased significantly (P<0.01). On the 7th day after radiation, the thymus index of the Bushen Jiedu Prescription prevention group and the Bushen Jiedu Prescription prevention and treatment group were significantly higher than the radiation model group (P<0.05 or P<0.01). On the 14th day after radiation, the thymus and spleen mass of Bushen Jiedu Prescription prevention group and Bushen Jiedu Prescription prevention and treatment group were higher than the amifostine positive control group and the radiation model group (P<0.01 or P<0.05). (2) On the 1st day after radiation, compared with the blank control group, the white blood cells and lymphocytes of each group were significantly decreased (P<0.01). On the 7th day after radiation, the number of lymphocytes in the Bushen Jiedu Prescription prevention and treatment group and Bushen Jiedu Prescription prevention group was significantly larger than that of the amifostine positive control group (P<0.05). On the 14th day after radiation, the white blood cells and lymphocytes of the mice in the Bushen Jiedu Prescription prevention and treatment group and Bushen Jiedu Prescription prevention group were significantly higher than those in the amifostine positive control group and the radiation model group (P<0.01 or P<0.05). Conclusion Bushen Jiedu Prescription can promote the recovery of thymus and spleen after radiation, increase the total number of peripheral blood leukocytes and lymphocytes, and has a good radiation protection effect. |
| Key words: Bushen Jiedu Prescription radiation immune organ immune cell |
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