| 引用本文: |
高飞,刘铁钢,徐竞男,白辰,姜欣,李旖旎,黄羚,梅沉成,于河,谷晓红.银莱汤对胃肠积热合并肺炎大鼠胃肠动力的作用及机制研究[J].湖南中医药大学学报,2019,39(3):302-306[点击复制] |
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| 银莱汤对胃肠积热合并肺炎大鼠胃肠动力的作用及机制研究 |
| 高飞,刘铁钢,徐竞男,白辰,姜欣,李旖旎,黄羚,梅沉成,于河,谷晓红 |
| (北京中医药大学中医学院, 北京 100029) |
| 摘要: |
| 目的 制作胃肠积热合并肺炎模型，观察银莱汤对胃肠积热合并肺炎大鼠胃排空及小肠推进率的影响，通过检测血清中胃饥饿素（Ghrelin）、NO含量变化，初步探讨银莱汤调节胃肠动力的具体机制。方法 将实验大鼠分为正常组、胃肠积热组、胃肠积热合并肺炎组和银莱汤治疗组共4组，每组10只。其中胃肠积热组、胃肠积热合并肺炎组和银莱汤治疗组给予高热量饲料喂养，并结合52%牛奶溶液灌胃的方法制作食积动物模型，且后2组采用0.5 mg/mL LPS吸入雾化处理，并予以生理盐水或银莱汤灌胃相应的干预。采集大鼠宏观表征，行胃排空和小肠推进实验，并用Elisa法测定各组大鼠血清中Ghrelin、NO含量。结果 与正常组相比，胃肠积热组大鼠的活动度较差，摄食量减少，体质量显著降低（P<0.05）；与胃肠积热合并肺炎组相比，银莱汤治疗组大鼠胃排空和小肠推进率明显增加（P<0.05）；与胃肠积热合并肺炎组相比，银莱汤治疗组大鼠血清的Ghrelin有所升高，且NO含量明显降低（P<0.05）。结论 胃肠积热和肺炎因素会影响胃肠道内相关激素的分泌，从而导致其胃排空和小肠推进率的异常，而银莱汤能够通过升高血清中Ghrelin含量，并能抑制NO的激素水平，来达到促进胃肠动力作用。 |
| 关键词: 银莱汤 胃肠积热 肺炎 胃饥饿素 一氧化氮 肺胃同治 |
| DOI：10.3969/j.issn.1674-070X.2019.03.004 |
| 投稿时间：2018-07-06 |
| 基金项目:国家自然科学基金项目（81373769）；北京市科技新星项目（Z181100006218083）；中央高校基本科研业务费专项资金（2018-JYBXS） |
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| Effect of Yinlai Decoction on Gastrointestinal Motility in Rats with Accumulated Heat in Stomach and Intestine with Pneumonia and Its Mechanism |
| GAO Fei,LIU Tiegang,XU Jingnan,BAI Chen,JIANG Xin,LI Yini,HUANG Ling,MEI Chencheng,YU He,GU Xiaohong |
| (School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China) |
| Abstract: |
| Objective To observe the effects of Yinlai Decoction on gastric emptying and small intestine propulsion rate in rats with accumulated heat in stomach and intestine (AHSI) with pneumonia by establishing a rat model of AHSI with pneumonia, and to preliminary investigate the specific mechanism of Yinlai Decoction regulating gastrointestinal motility by determining the changes in serum levels of ghrelin and nitric oxide (NO). Methods A total of 40 experimental rats were equally and randomly divided into four groups:normal group, AHSI group, AHSI with pneumonia group, and Yinlai Decoction treatment group. The rats in the AHSI group, AHSI with pneumonia group, and Yinlai Decoction treatment group were given high-calorie feed and 52% milk solution to establish a rat model of dyspepsia. The rats with pneumonia were given 0.5 mg/ml lipopolysaccharide by aerosol inhalation and Yinlai Decoction or normal saline by gavage. The macroscopic characterization of rats was performed. The gastric emptying and small intestinal propulsion experiments were carried out in the rats, and the serum levels of ghrelin and NO were measured by enzyme-linked immunosorbent assay. Results Compared with the normal group, the AHSI group had significantly lower range of motion, food intake, and body weight (P<0.05). Compared with the AHSI with pneumonia group, the Yinlai Decoction treatment group had significantly higher gastric emptying rate, small intestine propulsion rate, and serum level of ghrelin (P<0.05) and a significantly lower serum level of NO (P<0.05). Conclusion AHSI and pneumonia may affect the secretion of relevant hormones in the gastrointestinal tract, resulting in abnormal gastric emptying and small intestine propulsion. Yinlai Decoction can increase the serum level of ghrelin and reduce the serum level of NO to promote gastrointestinal motility. |
| Key words: Yinlai Decoction accumulated heat in stomach and intestine pneumonia ghrelin nitric oxide therapy for lung and stomach |
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