| 引用本文: |
李盼,武才琪,王庆林.中草药日本鸢尾内生真菌的分离及其初步鉴定[J].湖南中医药大学学报,2019,39(2):198-202[点击复制] |
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| 中草药日本鸢尾内生真菌的分离及其初步鉴定 |
| 李盼,武才琪,王庆林 |
| (湖南师范大学医学院, 湖南 长沙 410013) |
| 摘要: |
| 目的 分析日本鸢尾（Iris japonica）内生真菌的类群分布。方法 通过菌落形态特征、显微镜下的孢子观察及基于ITS序列分析的分子生物学鉴定分析日本鸢尾各部位内生真菌的类群组成。结果 从日本鸢尾的根茎叶中共分离得到5种内生真菌。根中分离得到的内生真菌为橘青霉（Penicillium citrinum）、尖孢镰刀菌（Fusarium oxysporum），茎中为小新壳梭孢菌（Neofusicoccum parvum），叶中为橘青霉（Penicillium citrinum）、肉色隔孢伏革菌（Peniophora incarnata）、首都叶点霉（Phyllosticta capitalensis）。结论 自日本鸢尾分离获得5种内生真菌，其分布具有一定的组织特异性，可为药用植物内生真菌的开发和利用提供一定基础。 |
| 关键词: 日本鸢尾 内生真菌 分离 鉴定 |
| DOI：10.3969/j.issn.1674-070X.2019.02.011 |
| 投稿时间：2018-08-13 |
| 基金项目:湖南省基础医学重点学科基金资助（10A073） |
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| Isolation and Primary Identification of Endophytic Fungi from the Chinese Herbal Medicine Iris japonica |
| LI Pan,WU Caiqi,WANG Qinglin |
| (Medical College, Hunan Normal University, Changsha, Hunan 410013, China) |
| Abstract: |
| Objective To investigate the phylogenetic distribution of endophytic fungi from Iris japonica. Methods Colony morphological features, microscopic observation of spores, and molecular biological identification based on ITS sequence analysis were performed to analyze the composition of endophytic fungi at various parts of Iris japonica. Results Five species of endophytic fungi were isolated from the root, stem, and leaf of Iris japonica. Penicillium citrinum and Fusarium oxysporum were isolated from the root, Neofusicoccum parvum was isolated from the stem, and Penicillium citrinum, Peniophora incarnate, and Phyllosticta capitalensis were isolated from the leaf. Conclusion Five species of endophytic fungi are isolated from Iris japonica and their distribution has certain tissue specificity, which provides a basis for the development and utilization of endophytic fungi from medicinal plants. |
| Key words: Iris japonica endophytic fungus isolation identification |
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