| 引用本文: |
李强,黄杰,雷思敏,肖榕,吴萍,廖端芳,林艳.基于阴虚大鼠模型的玉竹有效部位筛选及其理化性质分析[J].湖南中医药大学学报,2019,39(1):35-39[点击复制] |
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| 基于阴虚大鼠模型的玉竹有效部位筛选及其理化性质分析 |
| 李强,黄杰,雷思敏,肖榕,吴萍,廖端芳,林艳 |
| (湖南中医药大学药学院, 湖南 长沙 410208;湘产大宗药材品质评价湖南省重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨玉竹干预阴虚大鼠模型的有效部位，分离纯化玉竹多糖（Polygonatumodoratum Polysaccharides，POP），并对其基本理化性质进行分析。方法 制备玉竹水总提取物（Ⅰ）、醇沉上清液（Ⅱ）和醇沉淀（Ⅲ）3个部位，大鼠腹腔注射三碘甲状腺原氨酸（T3），每日1次，连续1周，建立阴虚大鼠模型。检测各部位对阴虚大鼠体质量及体温变化的影响，采用Elisa法检测大鼠血清游离三碘甲状腺原氨酸（fT3）、游离四碘甲状腺原氨酸（fT4）、血浆环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）水平。采用DEAE-52纤维素柱层析对POP进行分离纯化，并对各均一多糖进行理化性质分析。结果 玉竹各部位均能显著增加阴虚大鼠的体质量，降低阴虚大鼠体温，对阴虚大鼠血清fT3、fT4、血浆cAMP、cGMP水平紊乱调节作用显著（P<0.01），且所含多糖的醇沉淀（Ⅲ）部位效果最佳。POP中分离得到1种中性多糖（POP1）和2种酸性多糖（POP2、POP3），其中POP1含量最高。结论 以中性多糖为主的玉竹多糖具有最佳滋阴药效，该研究为玉竹多糖的进一步开发提供了基础。 |
| 关键词: 玉竹 阴虚大鼠模型 有效部位 玉竹多糖 |
| DOI：10.3969/j.issn.1674-070X.2019.01.009 |
| 投稿时间：2018-06-22 |
| 基金项目:国家中医药行业专项（201507002）；湖南省重点研发计划项目（2017SK2125）；湖南省研究生省级科研创新项目（CX2015B336）；研究生创新课题（2017CX26）。 |
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| Screening for Active Fractions of Polygonatum odoratum in a Rat Model of Yin Deficiency and Analysis of Their Physicochemical Properties |
| LI Qiang,HUANG Jie,LEI Simin,XIAO Rong,WU Ping,LIAO Duanfang,LIN Yan |
| (School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the active fractions of Polygonatum odoratum in a rat model of Yin deficiency, to extract and purify Polygonatum odoratum polysaccharides (POPs), and to analyze their physicochemical properties.Methods Three fractions were prepared from Polygonatum odoratum, including the whole-plant aqueous extract (POP1), and supernatant (POP2) and precipitate (POP3) from ethanol precipitation. The rat model of Yin deficiency was established by intraperitoneal injection of triiodothyronine (T3) once a day for 1 week. The effect of the three fractions on the changes in body weight and temperature of the rats with Yin deficiency was evaluated. The levels of free triiodothyronine (FT3) and free tetraiodothyronine (FT4) in serum, and cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in plasma were measured by ELISA. POPs were extracted and purified by DEAE-cellulose-52 chromatography, and the physicochemical properties of each homogeneous polysaccharide were analyzed.Results All the fractions showed a significant effect in increasing the body weight, reducing the temperature, and regulating the serum levels of FT3 and FT4 and plasma levels of cAMP and cGMP in the rats with Yin deficiency (P<0.01), with the best effect observed in POP 3. One type of neutral polysaccharide (POP1) and two types of acidic polysaccharides (POP2 and POP3) were extracted from POPs, among which the content of POP1 was highest.Conclusion The best Yin-nourishment effect is observed in the neutral polysaccharide-dominated Polygonatum odoratum polysaccharide. This study provides a basis for the further development of Polygonatum odoratum polysaccharides. |
| Key words: Polygonatum odoratum rat model of Yin deficiency active fraction Polygonatum odoratum polysaccharide |
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