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刘文娥,张婉妮,周意,陈青.盆炎丸对小鼠解脲支原体感染生殖器组织学变化的实验研究[J].湖南中医药大学学报,2018,38(10):1110-1114[点击复制] |
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| 盆炎丸对小鼠解脲支原体感染生殖器组织学变化的实验研究 |
| 刘文娥,张婉妮,周意,陈青 |
| (湖南中医药大学第一附属医院妇产科, 湖南 长沙 410007;湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院感染科, 湖南 长沙 410007) |
| 摘要: |
| 目的 观察盆炎丸对解脲支原体（ureaplasma urealyticum，UU）感染小鼠组织形态学的影响，初步探讨盆炎丸对UU感染的治疗机制。方法 采用皮下注射苯甲酸雌二醇，阴道接种解脲支原体标准菌株的方法，制备UU感染小鼠模型，造模组造模成功后随机分为中药组、西药组、模型组，另设空白组，前2组分别予以盆炎丸、克拉霉素，后2组予以等容量生理盐水灌胃，连续2周后观察小鼠一般情况、生殖器外观及镜下阴道、宫颈组织形态学及图像分析炎症细胞比例。结果 模型组小鼠一般情况欠佳，外阴溃烂明显，溃烂面积大，阴道分泌物明显增加；中药组、西药组小鼠一般情况可，阴道分泌物增加，外阴红肿，部分可见溃烂，面积较小。模型组小鼠阴道、子宫充血水肿，宫颈肥大，输卵管积水，增粗，弹性差，僵硬，个别出现盆腔粘连；中药组以疏松水肿为主，西药组充血水肿，程度较轻。镜下观察：模型组小鼠可见阴道黏膜、间质层不同程度中性粒细胞浸润，黏膜层、间质明显疏松水肿，宫颈腺体扩张或增生，黏膜及黏膜下组织可见嗜酸性粒细胞及浆细胞浸润。与模型组相比，中药组阴道组织及宫颈组织炎症明显消退，炎症细胞比例差异均有统计学意义（P<0.01）。结论 盆炎丸可改善动物一般情况，减轻UU感染小鼠阴道组织损伤程度及宫颈组织炎症。 |
| 关键词: 盆炎丸 解脲支原体 阴道 宫颈 组织形态 |
| DOI：10.3969/j.issn.1674-070X.2018.10.004 |
| 投稿时间：2016-03-21 |
| 基金项目:湖南省自然科学基金项目（2015JJ4040）；湖南省科技厅项目（2012TT2024）；湖南省中医药管理局项目（2016103）。 |
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| Effect of Penyan Pill on Changes in Genital Histology in Mice Infected with Ureaplasma urealyticum |
| LIU Wen'e,ZHANG Wanni,ZHOU Yi,CHEN Qing |
| (Department of Obstetrics and Gynecology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Department of Infectious Diseases, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To observe the effect of Penyan Pill on genital histomorphology in mice infected with Ureaplasma urealyticum (UU), and to preliminarily investigate the therapeutic mechanism of Penyan Pill on UU infection. Methods A UU-infected mouse model was established by subcutaneous injection of estradiol benzoate and vaginal inoculation of standard UU strain. The model mice were randomly divided into traditional Chinese medicine (TCM) group, Western medicine group, and model group, and a blank group was set; these mice were given Penyan Pill, clarithromycin, the same volume of saline, and the same volume of saline by gavage, respectively. After two weeks of treatment, the general status, genital appearance, and the microscopic morphology of the vagina and cervical tissue were observed, and the proportions of inflammatory cells were analyzed by Motic image analysis software. Results Compared with the blank group, the model group had a poor general status, obvious and large vulva ulceration, and significantly increased vaginal discharge; the TCM group and the Western medicine group had a fair general status, increased vaginal discharge, red and swollen vulva, and small ulceration in some vulvas. Compared with the blank group, the model group had hyperemia and edema of the vagina and uterus, cervical hypertrophy, hydrosalpinx, enlargement, poor elasticity, and stiffness of the fallopian tube, and pelvic adhesion in some mice; the TCM group mainly had loosening and edema of the vagina and uterus; the Western medicine group had mild hyperemia and edema of the vagina and uterus. Compared with the blank group, the model group had varying degrees of neutrophil infiltration in the vaginal mucosa and mesenchyme, obviously loose and swollen mucous membrane and mesenchyme, enlargement or hyperplasia of the cervical gland, and eosinophils and plasma cell infiltration in the cervical mucosal and submucosal tissue. Compared with the model group, the TCM group had significantly obvious inflammation resolution in the vaginal tissue and cervical tissue (P<0.01). There were significant differences in the proportions of inflammatory cells between the model group and the TCM group (P<0.01). Conclusion Penyan Pill can improve general status and reduce damage of the vaginal tissue and inflammation of the cervical tissue in UU-infected mice. |
| Key words: Penyan Pill Ureaplasma urealyticum vagina cervix histomorphology |
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