| 引用本文: |
刘培,肖隋熙,罗颖,陈偶英,刘天洋,郭心鸽,谭元生,简维雄.自发性高血压大鼠左心室肥厚的蛋白质谱研究[J].湖南中医药大学学报,2018,38(6):605-613[点击复制] |
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| 自发性高血压大鼠左心室肥厚的蛋白质谱研究 |
| 刘培,肖隋熙,罗颖,陈偶英,刘天洋,郭心鸽,谭元生,简维雄 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 研究自发性高血压大鼠（spontaneously hypertensive rats，SHR）左心室肥厚心肌组织的蛋白质变化。方法 对8只SHR与8只WKY大鼠组织蛋白质进行iTRAQ检测分析；通过对差异蛋白Pathway富集分析选择与通路相关的蛋白质。结果 筛选出与通路相关的40个差异蛋白，SHR与WKY比较上调的差异蛋白有：1主要组织相容性复合物、2血小板活化因子乙酰水解酶、3有丝分裂原蛋白激酶、4热休克27kDa蛋白、5 Ighg3、6 14-3-3γ蛋白、7纤维蛋白原、8蛋白酶体26S、9纤维蛋白原γ链、10中性胆固醇酯水解酶、11溶血PAF乙酰转移酶、12纤连蛋白。下调的差异蛋白有：13丝氨酸蛋白酶、14载脂蛋白H、15血清锌α2糖蛋白、16泛醌9、17泛醌7、18α2-AP、19激肽原、20肝素2、21未知蛋白、22钠钾ATP转移酶、23胶原蛋白、24集聚蛋白、25对羟基苯丙酮酸双氧化酶、26C反应蛋白、27整合素、28凝血因子Ⅻ、29胎球蛋白、30整合素α7、31碳酸酐酶、32CD36、33免疫球蛋白重链γ多肽、34转胶蛋白、35血红蛋白-α。结论 SHR左心室肥厚发生涉及心肌凋亡、细胞增殖增生、炎症等多种细胞因子，导致细胞损伤与保护作用失衡。 |
| 关键词: 自发性高血压大鼠 左心室肥厚 蛋白质组学 |
| DOI：10.3969/j.issn.1674-070X.2018.06.001 |
| 投稿时间：2017-12-29 |
| 基金项目:国家自然科学基金项目（81774207；81202647；81473616），中医诊断国家重点学科开放项目（2014-16）。 |
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| Proteomic Analysis of Left Ventricular Hypertrophy in Spontaneously Hypertensive Rats |
| LIU Pei,XIAO Suixi,LUO Ying,CHEN Ouying,LIU Tianyang,GUO Xinge,TAN Yuansheng,JIAN Weixiong |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To study the protein changes in the myocardium of left ventricular hypertrophy in spontaneously hypertensive rats (SHR). Methods The protein of 8 SHR and 8 WKY rats were detected and analyzed by iTRAQ detection and analysis technique. The proteins related to pathway were selected by enrichment analysis of differential protein pathway. Results The 40 differential proteins related to pathways were screened. Upregulated proteins in SHR compared with WKY:1 major histocompatibility complex, 2 platelet activating factor acetylhydrolase, 3 mitogen activated protein kinase, 4 heat shock protein 27 kDa, 5 Ighg 3, 6 14-3-3 protein gamma, 7 ibrinogen, 8 fibrin and 26 S proteasome, 9 the original gamol/La chain, 10 neutral cholesteryl ester hydrolase, 11 hemolytic PAF acetyltransferase, 12 fibronectin. Down regulated differentially proteins:13 serine protease, 14 apolipoprotein H, 15 serum zinc alpha 2 glycoprotein, 16 ubiquinone 9, 17 ubiquinone 7, 18 alpha 2-AP, 19 kininogen, 20 heparin cofactor 2, 21 unknown protein, 22 sodium-potassium ATP transferase, 23 collagen, 24 agrin, 25 concentration of HPPD, 26 C reactive protein, 27 integrin, 28 coagulation factor XⅡ, 29 fetuin 30 integrin alpha 7, 31 carbonic anhydrase, 32 CD36, 33 immune globulin heavy chain gamma polypeptide, 34 transgelin, 35 hemoglobin alpha. Conclusion Left ventricular hypertrophy in SHR involves many kinds of cytokines, such as myocardial apoptosis, cell proliferation, proliferation, inflamol/Lation and so on, leading to the imbalance of cell injury and protection. |
| Key words: spontaneously hypertensive rats left ventricular hypertrophy proteomics |
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