| 引用本文: |
刘李玟韬,杨洁,陈秉朴.锌-α2-糖蛋白在高尿酸血症模型小鼠肾脏中表达的变化[J].湖南中医药大学学报,2018,38(4):384-388[点击复制] |
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| 锌-α2-糖蛋白在高尿酸血症模型小鼠肾脏中表达的变化 |
| 刘李玟韬,杨洁,陈秉朴 |
| (右江民族医学院基础医学院, 广西 百色 533000;右江民族医学院附属医院, 广西 百色 533000) |
| 摘要: |
| 目的 以高尿酸血症模型小鼠为研究对象，探讨血清尿酸水平与肾脏组织中锌-α2-糖蛋白（ZAG）的相关性，以寻求防治高尿酸血症的新靶点。方法 将20只SPF级昆明小鼠随机分为模型组和空白对照组，97%氧嗪酸钾盐按0.5 g/（kg·d）腹腔注射，建立高尿酸血症小鼠模型，造模7 d后，尾静脉采血检测血清尿酸（SUA）水平确定模型建立成功，利用Western Blotting及实时荧光定量PCR技术检测肾脏中ZAG mRNA及蛋白水平。结果 与空白组相比，模型组小鼠肾脏组织ZAG mRNA及蛋白表达水平升高，差异均有统计学意义（P<0.05）。结论 血清尿酸可能影响肾脏ZAG的水平，但是ZAG与尿酸互相影响的具体机制仍需进一步研究。 |
| 关键词: 锌-α2-糖蛋白 高尿酸血症 肾脏 小鼠 |
| DOI：10.3969/j.issn.1674-070X.2018.04.005 |
| 投稿时间：2018-01-19 |
| 基金项目:广西高校科学技术研究项目（ZD2014105）；广西高校少数民族人类学重点研究室项目[桂教科研（2015）5号]。 |
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| Expression of Zinc-α2-glycoprote in the Kidney of Mice with Hyperuricemia |
| LIU Liwentao,YANG Jie,CHENG Bingpu |
| (Basic Medical School, Youjiang Medical College for Nationalities, Baise, Guangxi 533000, China;The Affiliated Hospital of Youjiang Medical College for Nationalities, Baise, Guangxi 533000, China) |
| Abstract: |
| Objective To explore the correlation between serum uric acid and kidney zinc-α2-glycoprotein(ZAG) in hyperuricemia mice model, in order to seek a new potential target for prevention and treatment of hyperuricemia. Methods 20 SPF Kunming mice were randomly divided into hyperuricemia model group and control group, 97% sylvite acid potassium salt of 0.5 g/(kg·d) was injected into the abdominal cavity. The hyperuricemia mice model was confirmed by the serum uric acid (SUA) level 7 days later. The levels of ZAG mRNA and protein were detected by Western blotting and real-time fluorescence quantitative. Results Compared with the blank group, the levels of ZAG mRNA and protein in the kidney tissue in the model group increased, the difference was statistically significant (P<0.05). Conclusion Serum uric acid may affect the level of ZAG, and the correlation mechanism between ZAG and uric acid needs to be further explored. |
| Key words: zinc-α2-glycoprotein hyperuricemia kidney mice |
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