| 引用本文: |
颜虹,贺平,武姿含,蒋素容,田浩梅,陈楚淘.针刺大椎、百会、人中穴对大鼠脑缺血再灌注损伤后不同时间点海马脑红蛋白表达的影响[J].湖南中医药大学学报,2018,38(2):165-168[点击复制] |
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| 针刺大椎、百会、人中穴对大鼠脑缺血再灌注损伤后不同时间点海马脑红蛋白表达的影响 |
| 颜虹,贺平,武姿含,蒋素容,田浩梅,陈楚淘 |
| (湖南中医药大学针灸推拿学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察针刺大椎、百会、人中穴对大鼠脑缺血再灌注损伤后不同时间点海马脑红蛋白（neuroglobin，Ngb）表达的影响，探讨其脑保护作用的部分作用机制。方法 采用线栓法制备MCAO模型，将40只雄性SD大鼠随机分为假手术组、模型组、对照点组、穴位组4大组，每组再根据再灌注后时间分为24 h、72 h组，每组5只。完成治疗后，先行神经功能缺损评分再处死大鼠，再采用Western Blot法检测大鼠缺血侧海马Ngb的表达水平。结果 神经功能缺损评分：与假手术组比较，模型组、对照点组及穴位组神经功能缺损评分均升高，差异有统计学意义（P<0.05，P<0.01）；模型组、对照点组、穴位组三组神经功能缺损评分差异无统计学意义（P>0.05）。与24 h组比较，72 h模型组神经功能缺损评分差异无统计学意义（P>0.05）；72 h对照点组及穴位组神经功能缺损评分均下降，差异有统计学意义（P<0.05）。Ngb：与假手术组比较，模型组Ngb的表达水平明显降低（P<0.01）；与模型组比较，对照点组及穴位组Ngb的表达水平均明显升高（P<0.01）；与对照点组比较，穴位组Ngb的表达水平有升高，但差异无统计学意义（P>0.05）。与24 h组比较，72 h假手术组、模型组及对照点组Ngb的表达水平均有下降，但差异无统计学意义（P>0.05）；72 h穴位组Ngb的表达水平下降明显，差异有统计学意义（P<0.05）。结论 针刺能降低脑缺血再灌注损伤大鼠神经功能缺损评分，并可上调海马Ngb的表达水平，从而实现脑保护作用。 |
| 关键词: 脑缺血再灌注 针刺 大椎 百会 人中 海马组织 脑红蛋白 |
| DOI：10.3969/j.issn.1674-070X.2018.02.011 |
| 投稿时间：2017-11-01 |
| 基金项目:国家自然科学基金（81303051）；湖南省自然科学基金（2016JJ3101）；中医内科学省部共建教育部重点实验室（ZYNK201501）。 |
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| Effect of Acupuncturing Dazhui, Baihui and Renzhong Acupoints on Expression of Neuroglobin at Different Time Points in Hippocampus of Rats with Cerebral Ischemia Reperfusion Injury |
| YAN Hong,HE Ping,WU Zihan,JANG Surong,TIAN Haomei,CHEN Chutao |
| (College of Acupuncture and Massage, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of acupuncturing Dazhui, Baihui and Renzhong acupoints on hippocampus neuroglobin (Ngb) expression at different time points in rats with middle cerebral artery occlusion (MCAO), and to explore the part mechanism of brain protection.Methods MCAO model was established by thread embolism method. 40 male SD rats were randomly divided into 4 groups:sham operation group, model group, control point group and acupoint group. Each group was then divided into two groups according to reperfusion time at 24 h and 72 h, 5 rats in each group. After treatment, the nerve function defect score was measured, then the rats were executed. The expression of Ngb in the ischemic side of hippocampus of rats was determined by Western Blot.Results The neurological deficit score:compared with sham operation group, nerve function defect in model group, control group and acupuncture group increased, the difference was statistically significant (P<0.05, P<0.01), while neural function defect score between the model group, control group and acupuncture group was not statisitically significant (P>0.05). There was no significant difference in neurological deficit scores at 24 h and 72 h of model group (P>0.05). The neurological deficit scores at 72 h in control group and acupoint group all decreased, the difference was statistically significant (P<0.05). Ngb:compared with the sham group, the expression level of Ngb in the model group decreased significantly (P<0.01); compared with the model group, the expression of Ngb in the control group and acupuncture group significantly increased (P<0.01); and compared with the control group, the expression of Ngb in acupuncture group increased, but the difference was not statistically significant (P>0.05). Compared with the time at 24 h, the expression level of Ngb at 72 h in sham operation group, model group and control group decreased, but the difference was not statistically significant (P>0.05); the expression level of brain erythroprotein at 72 h in acupuncture group decreased significantly, the difference was statistically significant (P<0.05).Conclusion Acupuncture could reduce the neurological deficit score in rats with cerebral ischemia-reperfusion injury, and up-regulate the expression level of erythromyin in hippocampus to achieve the protective effect on brain. |
| Key words: cerebral ischemia reperfusion acupuncture Dazhui Baihui Renzhong hippocampus neuroglobin |
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