| 引用本文: |
欧阳荣键,路延之,周其全.参芪花粉片对低氧暴露下大鼠胃肠黏膜屏障损伤的保护作用观察[J].湖南中医药大学学报,2018,38(2):154-159[点击复制] |
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| 参芪花粉片对低氧暴露下大鼠胃肠黏膜屏障损伤的保护作用观察 |
| 欧阳荣键,路延之,周其全 |
| (中国人民解放军第三十七医院, 四川 雅安 625000;陆军军医大学新桥医院, 重庆 400037;空军军医大学基础部微生物教研室, 陕西 西安 710032;陆军军医大学高原军事医学系, 重庆 400038) |
| 摘要: |
| 目的 探讨低氧暴露下胃肠黏膜屏障损伤的机制及参芪花粉片对其产生的保护作用，为胃肠黏膜的应激损伤防治提供科学依据。方法 60只SD大鼠随机分为平原对照组（PCT）、单纯低氧暴露组（SHE）和低氧暴露+参芪花粉片保护组（GAP），在模拟海拔7 000 m的低压舱内暴露72 h，观察各组大鼠小肠黏膜厚度改变、肠上皮细胞凋亡、肠道细菌移位情况，检测血清和小肠的TNF-α和IL-6水平，以及TLR4 mRNA表达水平。结果 低氧暴露组大鼠肠黏膜损伤严重，紧密连接间隙增宽，硝酸镧颗粒进入上皮细胞紧密连接间隙内以及周围组织间隙或细胞内，肠系膜淋巴结和脾脏有细菌移位；低氧暴露+参芪花粉保护组肠黏膜损伤减轻，黏膜厚度增加，硝酸镧颗粒很少进入组织间隙和细胞内，移位细菌明显减少。与平原对照组比较，低氧暴露组血清内毒素、TNF-α和IL-6水平显著增高，参芪花粉片保护组血清内毒素、TNF-α和IL-6水平显著降低；低氧暴露后空肠组织TLR4 mRNA表达量显著增加，给予参芪花粉片后TLR4表达量明显下降，差异有统计学意义（P<0.01）。结论 低氧暴露能引起严重的肠黏膜屏障功能损伤，给予参芪花粉片后可以降低肠黏膜通透性，对肠黏膜损伤具有一定的保护作用。 |
| 关键词: 低氧暴露 肠黏膜 屏障损伤 细菌易位 参芪花粉片 |
| DOI：10.3969/j.issn.1674-070X.2018.02.009 |
| 投稿时间：2017-04-12 |
| 基金项目: |
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| Protective Effect of Shenqi Huafen Tablet on Gastrointestinal Mucosal Barrier Injury Under Hypoxic Exposure |
| OUYANG Rongjian,LU Yanzhi,ZHOU Qiquan |
| (The 37 th Hospital of PLA, Yaan, Sichuan 625000, China;Xinqiao Hospital, Army Military Medical University, Chongqing 400037, China;Department of Microbiology, Basic Medical College, Air Force Medical University, Xi'an, Shaanxi 710032, China;High Altitude Military Medicine College, Army Military Medical University, Chongqing 400038, China) |
| Abstract: |
| Objective To investigate the protective effect of oral Shenqi Huafen tablet on gastrointestinal mucosal barrier injury induced by hypoxia and provide scientific evidence for the prevention and treatment of various gastrointestinal mucosa stress injury.Methods Sixty Sprague dawley rats were randomly divided into plain control group(PCT), simple hypoxia exposure group (SHE) and hypoxia with Shenqi Huafen tablet protection group (HAP). The hypoxia treated rats were placed in a simulated altitude of 7 000 m in a hypobaric chamber for 72 hours, and the thickness changes of rat small intestine, intestinal epithelial cell apoptosis, bacterial translocation, level of TNF alpha and IL-6 levels in serum, level of TNF alpha and IL-6 levels in intestinal, as well the TLR4 mRNA expression in intestinal were observed.Results Rats in SHE group showed severe intestinal mucosal injury, the tight junction gap widened and lanthanum nitrates particles entered into the epithelial tight junctions and the surrounding interstitial space or within the cell. Mesenteric lymph nodes and spleen showed bacterial translocation. Compared with the PCT group, serum endotoxin and TNF-α and IL-6 levels increased significantly in SHE group, serum endotoxin and TNF-α and IL-6 significantly reduced in HGP group. The TLR4 mRNA expression rate increased significantly in Jejunum tissues in SHE group, while after giving with Shenqi Huafen tablet, the expression of TLR4 in intestine significantly decreased, the difference was statistically significant (P<0.01).Conclusion Hypoxia exposure could cause severe intestinal barrier dysfunction, promote bacterial and enterotoxin translocation. Shenqi Huafen tablet can decrease intestinal permeability, and shows a protective effect on intestinal mucosal injury. |
| Key words: hypoxia exposure intestinal mucosa barrier injury bacteria translocation Shenqi Huafen tablet |
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