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张雨林,谭洋,詹济华,满兴战,李雪,裴刚.芦笋提取物抗氧化和抑制3T3-L1前脂肪细胞分化活性研究[J].湖南中医药大学学报,2018,38(1):27-30[点击复制] |
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| 芦笋提取物抗氧化和抑制3T3-L1前脂肪细胞分化活性研究 |
| 张雨林,谭洋,詹济华,满兴战,李雪,裴刚 |
| (湖南中医药大学药学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 研究芦笋提取物的抗氧化能力和抑制3T3-L1前脂肪细胞分化的活性。方法 采用乙醇回流提取，通过酸碱处理，制备得到芦笋提取物；利用紫外分光光度法测定提取物中的酚酸含量（以没食子酸计）；采用DPPH法测定提取物清除自由基的活性；通过CCK8法及油红O染色法分别检测芦笋提取物对3T3-L1前脂肪细胞存活率及分化率的影响。结果 芦笋提取物中以没食子酸计的酚酸含量为0.5308 mg/mg；测得提取物清除自由基活性强弱与剂量成正相关，当其浓度达到2 mg/mL时，其对DPPH的清除率可达89.68%；在10~200μg/mL的测定浓度范围内，提取物对3T3-L1细胞生存率无明显影响，对3T3-L1细胞的分化有明显抑制作用，且呈剂量依赖性。结论 芦笋提取物具有较好清除自由基的活性，且活性强弱呈剂量依赖关系；芦笋提取物具有抑制前脂肪细胞分化的能力，且随剂量升高而增大。 |
| 关键词: 芦笋 酚酸 抗氧化 3T3-L1前脂肪细胞 |
| DOI：10.3969/j.issn.1674-070X.2017.01.007 |
| 投稿时间：2017-06-05 |
| 基金项目:湖南省教育厅科学研究项目（16K064）；长沙市科技局资助项目（kq1602015）；湖南省芦笋研究开发中心芦笋产业联合研究开发基金。 |
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| Study of the Activities of Antioxidant and Inhibiting Differentiation of 3T3-L1 Preadipocytes of the Extract from Phragmites australis (Cav.) Trin. ex Steud |
| ZHANG Yulin,TAN Yang,ZHAN Jihua,MAN Xingzhan,LI Xue,PEI Gang |
| (School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To study the activities of antioxidant and inhibiting differentiation of 3T3-L1 preadipocytes of Lusun[Phragmites australis (Cav.) Trin.ex Steud]extract.Methods The Lusun exatract was prepared by using ethanol reflux extraction and acid and alkali treatment.The content of phenolic acid of the extract was mearsured by UV spectrophotometry.The activity of free radical scavenging of extract was measured by using DPPH method.The effects of the extract on the survival rate and differentiation rate of 3T3-L1 preadipocytes were detected by CCK8 and oil red O staining.Results The content of phenolic acid in the Lusun extract was 0.5308 mg/mg calculated by gallic acid.The activity of scavenging free radicals of Lusun extract has positive correlation with dose,the DPPH removal rate was 89.68% when the concentration reached 2 mg/mL.The Lusun extract at the concentration of 10-200 μg/mL had no significant effect on the survival rate of 3T3-L1 cells,and had obvious inhibiting effect on the differentiation of 3T3-L1 cells in a dose-dependent manner.Conclusion The Lusun extract has significant scavenging free radical activity and have remarkable inhibition effect on 3T3-L1 cells proliferation,and the activities increase with the dose. |
| Key words: Phragmites australis (Cav.) Trin.ex Steud phenolic acid antioxidation 3T3-L1 preadipocytes |
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