| 引用本文: |
李路迢,刘旺华,李花,彭智远,周翠玲,付小金.健脾补土法组方含药血清对体外嗅鞘细胞低氧/复氧损伤模型P65、P50及磷酸化IκBα表达的影响[J].湖南中医药大学学报,2016,36(8):17-21[点击复制] |
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| 健脾补土法组方含药血清对体外嗅鞘细胞低氧/复氧损伤模型P65、P50及磷酸化IκBα表达的影响 |
| 李路迢,刘旺华,李花,彭智远,周翠玲,付小金 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨健脾补土法组方含药血清对体外嗅鞘细胞低氧/复氧损伤模型核因子KB（nuclear factor-kappa B，NF-KB）的亚单位P65、P50及NF-KB抑制蛋白（P-IκBα）表达的影响。方法 将原代培养的嗅鞘细胞分为5组：正常血清对照组、正常血清+低氧组、低氧+依达拉奉含药血清组、低氧+健脾补土法组方含药血清组、正常血清+低氧+NF-KB抑制剂/抗氧化剂（PDTC，50μmol/L）组，采用微需氧袋低氧/复氧的方法诱导嗅鞘细胞形成脑缺血再灌注损伤样模型，用Western blot法检测嗅鞘细胞胞浆P65、P50、P-IκBα和胞核P65、P50的含量。结果 与正常血清对照组比较，正常血清+低氧组胞浆和胞核P65、P50，胞浆P-IκBα表达均显著升高，差异有统计学意义（P<0.01）；与正常血清+低氧组比较，低氧+依达拉奉组、低氧+健脾补土组、正常血清+低氧+PDTC组、低氧+健脾补土+PDTC组胞浆和胞核P65、P50，胞浆P-IκBα表达均降低，差异有统计学意义（P<0.05）。结论 健脾补土法组方能通过抑制嗅鞘细胞缺氧/复氧损伤后P65、P50、P-IκBα的过度表达，对NF-κB通路的活化具有抑制作用。 |
| 关键词: 健脾补土法 嗅鞘细胞 低氧/复氧损伤模型 P65 P50 P-IκBα |
| DOI：10.3969/j.issn.1674-070X.2016.08.005 |
| 投稿时间：2016-03-18 |
| 基金项目:国家自然科学基金（81202632、81473567），教育部博士点基金（20124323120003），湖南省自然科学基金（13JJ3097），湖南省教育厅科研项目（14B134、15K092）。 |
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| Effect of Spleen-Strengthening Therapy Contained Serum on P65, P50 and P-IκBα Expression in Olfactory Ensheathing Cells with Hypoxia/Reoxygenation Injury Models |
| LI Lutiao,LIU Wanghua,LI Hua,PENG Zhiyuan,ZHOU Cuiling,FU Xiaojin |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effect of spleen-strengthening therapy contained serum on subgene P65,P50 of nudear factor-kappa B (NT-KB) and P-IκBα expression in olfactory ensheathing cells with hypoxia/reoxygenation injury.Methods Primary cultured olfactory ensheathing cells were randomly divided into five groups:normal serum control group,normal serum+hypoxia group,hypoxia+edaravone contained serum group,hypoxia+spleen-strengthening therapy contained serum group,normal serum+hypoxia+NF-KB inhibitor/antioxidant (PDTC 50 μmol/L) group.The cerebral ischemia-reperfusion injury model of olfactory ensheathing cells were induced by hypoxia/reoxygenation of micro-aerobic bags method.Then,the cytoplasm P65,P50,P-IκBα and nuclear P65,P50 content in olfactory ensheathing cells were detected by Western blot assay.Results Compared with normal serum control group,the cytoplasm and nucleus P65,P50,cytoplasm P-IκBα expression in normal serum+hypoxia group were significantly increased,the difference was statistically significant (P<0.01).Compared with normal serum control group,the cytoplasm and nucleus P65,P50,cytoplasm P-IκBα expression were lower in hypoxia+edaravone group,hypoxia+Jianpi Butu group,normal serum+hypoxia+PDTC group,hypoxia+Jianpi Butu+PDTC group,the difference was statistically significant (P<0.05).Conclusion Spleen-strengthening therapy can prevent the activation of NF-κB signal path,which may be due to its inhihitory effect on P65/P50 and P-IκBα overexpression in olfactory ensheathing cells with hypoxia/reoxygenation injury. |
| Key words: spleen-strengthening therapy olfactory ensheathing cells hypoxia/reoxygenation injury model P65 P50 P-IκBα |
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