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李岩岩,何泽云,吴凡,周艳利.六味地黄汤对5/6肾切除大鼠NF-κB、MCP-1、Col-Ⅲ表达的影响[J].湖南中医药大学学报,2016,36(5):18-22[点击复制] |
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| 六味地黄汤对5/6肾切除大鼠NF-κB、MCP-1、Col-Ⅲ表达的影响 |
| 李岩岩,何泽云,吴凡,周艳利 |
| (郑州市第三人民医院, 河南郑州 450000;湖南中医药大学第一附属医院, 湖南 长沙 410007;广州市中医医院, 广东 广州 510130) |
| 摘要: |
| 目的 观察六味地黄汤对5/6肾切除大鼠残肾转录因子-核因子κB（NF-κB）、单核细胞趋化因子（MCP-1）及Ⅲ型胶原（Col-Ⅲ）表达的影响，探讨六味地黄汤抑制肾间质纤维化进展的作用机制。方法 按照随机数字表法将60只SD雄性大鼠分为5组：空白组、假手术组、模型组、依那普利组、六味地黄汤组，每组12只，后三组行5/6肾切除术诱导大鼠肾衰模型，假手术组同期手术，但不损及肾脏。造模术后3 d进行灌胃干预。灌胃8周后处死各组大鼠，观察各组大鼠残肾组织形态学变化并用免疫组化法检测大鼠残肾NF-κB、MCP-1、Col-Ⅲ的表达。结果（1）观察5/6肾切除大鼠残肾组织细胞形态学，与模型组相比六味地黄汤、依那普利组可减轻大鼠肾间质损害及纤维化程度；（2）免疫组化半定量分析显示六味地黄汤组肾皮质的NF-κB、MCP-1及Col-Ⅲ表达均明显低于模型组（P<0.05），与依那普利组差异无统计学意义（P>0.05）。结论 六味地黄汤可能通过下调NF-κB、MCP-1及Col-Ⅲ的表达，减少细胞外基质的积聚，减轻炎症反应和纤维化程度，抑制5/6肾切除大鼠肾间质纤维化进展，可作为慢性肾衰竭的辅助用药。 |
| 关键词: 六味地黄汤 5/6肾切除 转录因子-核因子κB 单核细胞趋化因子 Ⅲ型胶原 |
| DOI：10.3969/j.issn.1674-070X.2016.05.005 |
| 投稿时间：2015-11-07 |
| 基金项目:湖南省科技厅项目资助（2013SK3099）。 |
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| Effects of Liuwei Dihuang Decoction on NF-κB, MCP-1 and Col-III Expression in 5/6 Nephrectomied Rats |
| LI Yanyan,HE Zeyun,WU Fan,ZHOU Yanli |
| (The Third Hospital of Zhengzhou, Zhengzhou, Henan 450000, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Guangzhou Hospital of TCM, Guangzhou, Guangdong 510130, China) |
| Abstract: |
| Objective To observe the effects of Liuwei Dihuang decoction on the expression of nuclear factor kappa B (NF-κB), monocyte chemoattractant protein-1(MCP-1)and collagen type III (Col-Ⅲ) in remnant kidney of 5/6 nephrectmized rats, and to investigate the mechanism of action for Liuwei Dihuang decoction on renal tubulointerstitial fibrosis. Methods The sixty male Sprapue-Dewley rats were randomly divided into five groups:blank group, sham-operation group, model group, enalapril group and Liuwei Dihuang decoction group, 12 rats in each group. The model group, enalapril group and Liuwei Dihuang decoction group adopted 5/6 nephrectomized induced into renal failure model. After 3 days of modeling, the rats were treated by gavage. After 8 weeks, rats were sacrificed, remnant kidney tissue morphological changes were observed and the expression of NF-kB, MCP-1, Col-Ⅲ levels were tested by immunohistochemistry. Results (1) The cell morphology in remnant kidney of 5/6 nephrectmized rats was observed. Compared with model group, renal tubulointerstitial damage and fibrosis was reduced in Liuwei Dihuang decoction and enalapril group; (2) Compared with model group, NF-κB, MCP-1 and Col-III expression level were reduced markedly in Liuwei Dihuang decoction group (P<0.05), there is no obvious diffrence between Liuwei Dihaung group and enalapril group (P>0.05). Conclusion Liuwei Dihuang decoction may inhibit the expression of NF-κB, reduce the expression of MCP-1, decrease the accumulation of extracellular matrix, reduce the inflammation and fibrosis and delay the progress of renal interstitial fibrosis, which can be the adjuvant drug of chronic renal failure. |
| Key words: Liuwei Dihuang decoction 5/6 nephrectomy nuclear factor kappa B monocyte chemoattractant protein-1 collagen type III |
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