| 引用本文: |
陈娥,周灿,廖莎,石金敏.不同炮制去心法对巴戟天耐斯糖含量的影响[J].湖南中医药大学学报,2016,36(4):31-33[点击复制] |
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| 不同炮制去心法对巴戟天耐斯糖含量的影响 |
| 陈娥,周灿,廖莎,石金敏 |
| (湖北医药学院附属人民医院药学部, 湖北 十堰 442000;湖北医药学院附属太和医院, 湖北 十堰 442000) |
| 摘要: |
| 目的 比较不同炮制去心法对巴戟天耐斯糖含量的影响。方法 采用HPLC-ELSD法，色谱柱为Welch AQ-C18（250 mm×4.6 mm，5 μm）；流动相为甲醇-水（3：97）；柱温为25℃；流速为0.8 mL/min；进样量为10 μL；漂移管温度为40℃；低温雾化。结果 采用不同炮制去心法处理后，巴戟天中耐斯糖的含量高低为：润法 > 煮法 > 清蒸法 > 盐蒸法 > 泡后蒸法 > 泡法。结论 采用润法去心，巴戟天中耐斯糖损失最少。 |
| 关键词: 巴戟天 炮制 耐斯糖 高效液相色谱-蒸发光散射检测器法 |
| DOI：10.3969/j.issn.1674-070X.2016.04.008 |
| 投稿时间：2015-09-06 |
| 基金项目:十堰市科学技术研究与开发项目计划（十科发[2013]20号）。 |
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| Influence of Different Methods for Removing Woody Core on Nystose Content in Morinda officinalis |
| CHEN E,ZHOU Can,LIAO Sha,SHI Jinmin |
| (Department of Pharmacy, Renmin Hospital Affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, China;Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, China) |
| Abstract: |
| Objective To compare the influence of different methods for removing woody core on nystose content in Morinda officinalis. Methods The HPLC-ELSD detection was used with the chromatographic column Welch AQ-C18 (250 mm×4.6 mm, 5 μm),the mobile phase was methanol-water(3:97),the column temperature was at 25℃,the flow rate was 0.8 mL/min,the injection volume was 10 μL, and the drift tube temperature was at 40℃ with low temperature atomization. Results The level of the nystose content in Morinda officinalis with different process was:moistening method > cooking method > steaming method > steaming method with salt > steaming method after soaking > soaking method. Conclusion The nystose content in Morinda officinalis is the least loss when use the moistening method to removing woody core. |
| Key words: Radix Morindae officinalis processing nystose HPLC-ELSD |
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