| 引用本文: |
张芳,刘建荣,马月宏,丁彩云,尹海珍,高嵩丹.精液中SCF/c-kit值在肾虚男性不育辨证中的价值[J].湖南中医药大学学报,2016,36(4):25-27[点击复制] |
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| 精液中SCF/c-kit值在肾虚男性不育辨证中的价值 |
| 张芳,刘建荣,马月宏,丁彩云,尹海珍,高嵩丹 |
| (山西省人民医院, 山西 太原 030012) |
| 摘要: |
| 目的 评价精液中干细胞因子（stem cell factor，SCF）及其受体（c-kit）在男性不育肾虚证中的临床诊断价值，为肾藏精主生殖的基本理论提供实验依据。方法 选取45例肾虚不育型患者与30例正常生育者进行对照研究，用酶联免疫吸附试验（ELISA法）测定两组精浆中SCF含量，用实时荧光定量PCR方法对精液离心沉淀物检测c-kit的ct值，并计算其相对表达量。结果 两组患者SCF浓度比较无差异（P>0.05），与正常生育组比较，肾虚不育患者前向运动精子数明显降低（P<0.01）、精液离心沉淀物c-kit相对表达量升高（P<0.05）。结论 男性不育肾虚证与精子活动和精液中c-kit表达量密切相关，两者可以作为男性不育肾虚证辨证时重要的客观指标，同时为中医肾藏精主生殖理论提供科学依据。 |
| 关键词: 男性不育 肾虚证 干细胞因子 原癌基因蛋白质 弱精 精液 |
| DOI：10.3969/j.issn.1674-070X.2016.04.006 |
| 投稿时间：2015-12-01 |
| 基金项目:山西省卫生厅科研课题（201201061）。 |
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| The Value of SCF/c-kit in Semen of Infertile Male with Nephrasthenia Syndrome |
| ZHANG Fang,LIU Jianrong,MA Yuehong,DING Caiyun,YIN Haizhen,GAO Songdan |
| (The People's Hospital of Shanxi, Taiyuan, Shanxi 030012, China) |
| Abstract: |
| Objective To evaluate the value of SCF/c-kit in the diagnosis for male infertility with kidney deficiency, and to provide experimental references of the theory to the kidney stores the essence and controls reproductivity. Methods 45 infertile males with kidney deficiency were selected and 30 fertile men were used as control. SCF in seminal plasma was determined by ELISA. The relative expression of c-Kit was detected by real-time PCR, and the relative expression quantity was caculated. Results There is no difference of SCF concentration between the two groups (P>0.05). Compared with fertile men, the forward movement of sperm in infertile men with kidney deficiency was decreased obviously (P<0.01), and relative expression of c-kit was significantly increased (P<0.05). Conclusion Kidney deficiency is closely related to sperm motility and the relative expression quantity of c-Kit in sperm., which can be as indicators for infertile male with kidney deficiency. This study provides scientific basis for the theory of kidney storing the essence and controlling reproductivity. |
| Key words: male infertility nephrasthenia syndrome stem cell factor c-kit asthenospermia semen |
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