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秦优,陈林,梁雪娟,糜亚男,肖娟,张水寒.雪峰虫草高效液相色谱指纹图谱研究[J].湖南中医药大学学报,2016,36(2):47-50[点击复制] |
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| 雪峰虫草高效液相色谱指纹图谱研究 |
| 秦优,陈林,梁雪娟,糜亚男,肖娟,张水寒 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省中医药研究院中药研究所, 湖南 长沙 410013;湖南省中医药研究院中药研究所, 湖南 长沙 410013;湖南省2011数字中医药协同创新中心, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208;湖南省中医药研究院中药研究所, 湖南 长沙 410013;湖南省2011数字中医药协同创新中心, 湖南 长沙 410208) |
| 摘要: |
| 目的 建立雪峰虫草药材的高效液相色谱指纹图谱，为评价其质量提供依据。方法 采用高效液相色谱法，色谱柱为月旭AQ-C18（4.6 mm×250 mm，5 μm）；检测波长260 nm；以甲醇-0.1%甲酸水溶液为流动相进行梯度洗脱；体积流量0.5 mL/min；柱温30℃；进样量20 μL。结果 建立了11批雪峰虫草药材高效液相色谱指纹图谱，确定共有峰15个，采用对照品结合HPLC-Q-TOF-MS/MS技术对7个特征峰进行了指认，并采用相似度分析、主成分分析、聚类分析的方法对指纹图谱进行了分析。结论 建立的雪峰虫草高效液相指纹图谱方法简便、可靠且重复性好，为雪峰虫草的质量控制提供了有效的手段。 |
| 关键词: 雪峰虫草 高效液相色谱 指纹图谱 质量控制 聚类分析度 相似度分析 |
| DOI：10.3969/j.issn.1674-070X.2016.02.013 |
| 投稿时间：2015-10-21 |
| 基金项目:湖南省科技重大专项（2014FJ1007）；湖南省中医药科研计划项目（201267；201322）；中医药公共卫生专项“国家基本药物所需中药原料资源调查和监测项目”[财社（2011）-76]。 |
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| HPLC Fingerprint of Ophiocordyceps xuefengensis |
| QIN You,CHEN Lin,LIANG Xuejuan,MI Yanan,XIAO Juan,ZHANG Shuihan |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Academy of Chinese Medicine, Changsha, Hunan 410013, China;Hunan Academy of Chinese Medicine, Changsha, Hunan 410013, China;2011 Collaboeation and Innovation Center for Digital Chinese Medicine in Hunan, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Academy of Chinese Medicine, Changsha, Hunan 410013, China;2011 Collaboeation and Innovation Center for Digital Chinese Medicine in Hunan, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To establish HPLC fingerprint for Ophiocordyceps xuefengensis and provide conference for quality evaluation. Methods The HPLC method was used with the condition that the column was Yuexu AQ-C18 (4.6 mm×250 mm, 5 μm); The detection wavelength was set at 260 nm; The mobile phase was eluted with gradient by mixture of methanol and water (0.1% formic acid); The flow rate was 0.5 mL/min; The column temperature was 30℃; The injection was 20 μL. Results The HPLC fingerprint for Ophiocordyceps xuefengensis collected from eleven different sources was set up. Fifteen common peaks werefound, seven of them had been identified by reference substances and HPLC-Q-TOF-MS/MS. The fingerprint was analyzed with methods of cluster analysis, principal component analysis and similarity computation. Conclusion The method of HPLC fingerprint for Ophiocordyceps xuefengensis is simple, rapid and can be used for identification and evaluation of Ophiocordyceps xuefengensis. |
| Key words: Ophiocordyceps xuefengensis HPLC fingerprints quality control cluster analysis simillarity analysis |
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