| 引用本文: |
祁芳,李艳玲,艾坤,蔡雄,李鑫,刘梨,张泓,杨茜芸.SD大鼠佐剂性关节炎模型的建立与评估[J].湖南中医药大学学报,2016,36(1):23-26[点击复制] |
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| SD大鼠佐剂性关节炎模型的建立与评估 |
| 祁芳,李艳玲,艾坤,蔡雄,李鑫,刘梨,张泓,杨茜芸 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 通过在不同组的SD大鼠尾部注射不同剂量的结核分枝杆菌H37Ra（Mtb）溶液，探索最佳致炎剂量，为类风湿关节炎大鼠的造模方法及基础研究提供理论依据。方法 采用灭活的Mtb与矿物油混匀，配制成浓度分别为0.625、1.25、2.5、5.0 mg/mL的佐剂，每组大鼠均给予相应浓度的MT悬浮液0.1 mL于尾根部皮下注射，诱导SD大鼠类风湿关节炎。在实验周期内，定期测量大鼠体质量，进行足爪容积测定、关节炎评分等，最后综合各项指标进行分析讨论，探讨得出最佳致炎剂量。结果 62.5 μg组不能充分诱导出关节炎，被剔除。125 μg组、250 μg组、500 μg组成功率均为100%，该3组关节肿胀程度、关节炎评分等无明显差异（P>0.05），其中125 μg组大鼠关节受累范围和关节肿胀程度均轻于250 μg组和500 μg组。结论 由Mtb诱导的佐剂性关节炎大鼠模型造模成功率高，最佳致炎剂量可能为125 mg/mL，0.1 mL/只。 |
| 关键词: SD大鼠 佐剂性关节炎 结核分枝杆菌H37Ra 致炎剂量 |
| DOI：10.3969/j.issn.1674-070X.2016.01.005 |
| 投稿时间：2015-11-14 |
| 基金项目:国家自然科学青年基金（81303048）；湖南省科技厅科技计划一般项目（2014SK3046）；湖南省教育厅科学研究优秀青年项目（15B176）；湖南省教育厅科学研究项目（14C0854）。 |
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| The Establishment and Evaluation of Adjuvant-Induced Arthritis in SD Rats |
| QI Fang,LI Yanling,AI Kun,CAI Xiong,LI Xin,LIU Li,ZHANG Hong,YANG Qianyun |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To explore the best dosage of establishing rheumatoid arthritis model (AIA) rats by caudal injection with different concentration of Mycobacterium tuberculosis H37Ra(Mtb) solution of different groups' SD rats to provide the theory basis for establishing AIA rats methods and its fundamental research. Methods Adopt the Heat-killed Mtb mix with mineral oil, the mixture concentration were 0.625, 1.25, 2.5, 5.0 mg/mL, respectively. the concentration of rats in each group are given the corresponding MT suspension 0.1 mL in the tail root subcutaneously to induce the rheumatoid arthritis. During the experimental period, the rats' body weight was measured at fixed period, the foot paw volume and arthritis were scored, etc, regularly. In the end, the most suitable dosage of inflammatory is selected based on the indexes being synthesized. Results The 62.5 μg can not induce the successful arthritis models and the group of 62.5 μg was removed. The 125 μg, 250 μg and 500 μg groups were all 100%, and there were no significant difference of degree of joint swelling, arthritis score, etc (P>0.05); and the arthritis size and joint swelling degree of rats in 125 μg group were lower than that in 250 μg and 500 μg groups. Conclusion The Mtb induced AIA rat models establishment has a high success rates, and the best inflammatory dosage is probably1.25 mg/mL, 0.1 mL per rat. |
| Key words: SD rats adjuvant arthritis Mycobacterium tuberculosis H37Ra inflammatory dosage |
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