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张运辉1,伍大华1*,袁春云1，张秀丽2，彭文杰1，姚 婷1.二苯乙烯苷和三七总皂苷配伍对Aβ25-35致PC12细胞损伤影响的研究[J].湖南中医药大学学报,2015,35(9):20-22[点击复制] |
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| 二苯乙烯苷和三七总皂苷配伍对Aβ25-35致PC12细胞损伤影响的研究 |
| 张运辉1,伍大华1*,袁春云1，张秀丽2，彭文杰1，姚婷1 |
| (1.湖南中医药大学中医药研究院附属中西结合医院，湖南 长沙 410006;2.湖南中医药大学中药粉体实验室，湖南 长沙 410208) |
| 摘要: |
| 目的 探讨二苯乙烯苷(TSG)和三七总皂苷(PNS)配伍对Aβ25-35诱导的PC12细胞损伤的影响。方法 PC12细胞采用Aβ25-35诱导建立阿尔茨海默病的损伤模型，被随机分成空白组、模型组、多奈哌齐组（10 μmol/L）、TSG（100 mmol/L）组、PNS组（50 mg/L）、TSG-PNS配伍组（TSG100 mmol/L+PNS50 mg/L)。取细胞上清液测各组的乙酰胆碱酯酶（AchE）及超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量。结果 与模型组比较，TSG组、PNS组、TSG-PNS配伍组可降低AchE活力、MDA含量及提高SOD活力(P<0.05)。且TSG-PNS配伍组的效应比TSG或PNS单用组效应更好(P<0.01)。结论 TSG和PNS配伍对Aβ25-35诱导的PC12细胞损伤具有明显的保护作用，其作用机制可能与胆碱能系统及氧化应激有关。 |
| 关键词: 阿尔茨海默病 二苯乙烯苷 三七总皂苷 PC12细胞 配伍 |
| DOI： |
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| 基金项目:湖南省自然科学基金资助项目（14JJ2140）。 |
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| Study of Compatibility of TSG and PNS on PC12 Cell Injury Induced by Aβ25-35 |
| Yun-Hui Zhang1, Da-Hua Wu1*, Chun-Yun Yuan1, Xiu-Li Zhang2, Wen-Jie Peng1, Ting Yao1 |
| (1.The Integrated Chinese and Western Hospital Affiliated to Hunan Academy of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410006, China;2. TCM Powder Laboratory, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effect of compatibility of TSG and PNS on PC12 cell injury induced by Aβ25-35. Methods PC12 cell induced by Aβ25-35 was established Alzheimer's disease damage models, and PC12 cells were randomly divided into blank group, model group, Donay Patsy group (10 μmol/L), TSG group (100 mmol/L), PNS group (50 mg/L), TSG-PNS group (TSG 100 mmol/L + PNS 50 mg/L).The acetylcholinesterase (AchE), catalase (SOD) activity and malondialdehyde (MDA) content in supernatant were measured. Results Compared with the model group, the TSG group, PNS group and TSG-PNS group can reduce the vigor of AchE and the content of MDA, and increase the activity of SOD(P<0.05). Moreover, the effect of TSG-PNS group was better than only using TSG or PNS (P<0.01). Conclusion The compatibility of TSG and PNS exhibited a significant protective effect on PC12 cell injury induced by Aβ25-35 and its mechanism may be related to the cholinergic system and oxidative stress. |
| Key words: Alzheimer's disease stilbene glycoside panax notoginseng saponins PC12 cell compatibility of medicines |
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