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尚芬芬； 丁青； 陆惠； 陈苗苗；.护卵汤配合补佳乐及安宫黄体酮对卵巢早衰模型小鼠卵巢Th17和Treg的影响[J].湖南中医药大学学报,2012,32(9):29-32[点击复制] |
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| 护卵汤配合补佳乐及安宫黄体酮对卵巢早衰模型小鼠卵巢Th17和Treg的影响 |
| 尚芬芬；丁青；陆惠；陈苗苗； |
| (湖南中医药大学) |
| 摘要: |
| 目的观察护卵汤配合补佳乐及安宫黄体酮对卵巢早衰(POF)模型小鼠卵巢Th17和Treg表达水平的影响。方法采用透明带多肽(PZP3)及弗氏完全佐剂(CFA)和弗氏不完全佐剂(IFA)建立POF模型小鼠,随机分为空白组、模型组、护卵汤+补佳乐+安宫黄体酮组(护补安组)、补佳乐+安宫黄体酮组(补安组)、护卵汤组,护卵汤分别给药对POF模型小鼠进行干预后,采用酶联免疫吸附(ELISA)法检测各组小鼠的血清促卵泡生成素(FSH)、雌二醇(E2)含量;逆转录-聚合酶链反应(RT-PCR)检测小鼠卵巢的Th17和Treg表达水平;光镜下观察各组卵巢组织形态学变化。结果与模型组比较,护补安组、补安组、护卵汤组对致衰小鼠有治疗作用,差异具有统计学意义(P<0.01);护补安组、补安组在降低小鼠血清FSH含量方面优于护卵汤组(P<0.05);但护卵汤组在降低小鼠卵巢Th17水平上优于补安组,差异具有统计学意义(P<0.05)。结论护卵汤配合补
佳乐及安宫黄体酮可通过降低小鼠卵巢Th17而升高小鼠卵巢Treg水平治疗卵
巢早衰模型小鼠,且在降低小鼠血FSH含量上优于单纯护卵汤组。 |
| 关键词: 卵巢早衰 护卵汤 Th17 Treg 小鼠 菟丝子 桑椹子 |
| DOI： |
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| 基金项目:常德市科技局重点项目(2011SK18) |
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| effects of Huluantang combined with progynovaand medroxyprogesterone acetate on the expressions of Th17 and Treg of ovarian in premature ovarian failure mice model |
| SHANG Fen-fen,DING Qing,LU Hui,CHEN Miao-miao |
| ((TCM
University of Hunan,Changsha,Hunan 410208,China)) |
| Abstract: |
| (Abstract〕 Objective To observe the effects of
Huluantang combined with progynova
and medroxyprogesterone acetate on the expressions of Th17 and
Treg of ovarian in premature
varian failure(POF) mice. Methods The mouse model of autoimmune
POF was established by in-
jetting the zona pellucida 3 peptide fragments (pZP3)
combined with complete Freund adjuvant
(CFA) and incomplete Freund adjuvant (IFA). After immunization,
the mice were randomly divided
into 5 groups as follows: blank group, modeling
group, progynova combined
with medroxyprogesterone group, Huluantang group and
Huluantang combined with progynova
and medroxyprogesterone acetate group. After treatment, follicle
stimulating hormone(FSH) and estra-
diol(EZ) in serum were detected by ELISA, the Th17 and
Treg expression levels in ovarian were
examined by RT-PCR, the ovarian morphology changes were observed
under light microscope. Re-
cults Compared with modeling group, there were therapeutic effects
on treating POF mice in Hulu-
antang combined with progynova and medroxyprogesterone
acetate group, progynova combined
with medroxyprogesterone acetate group and Huluantang group, the
differences were statistically sig-
nificant ( all P <0.01).In terms of二、Iucing serum FSH,
Huluantang combined with progynova
and medroxyprogesterone acetate group and progynova combined
with medroxyprogesterone group
were superior[()Huluantang group (all P<0.05).But in terms of二、
Iucing Th17 levels of ovarian,
Huluantang group was superior[()progynova combined with
medroxyprogesterone group (P<0.05).
Conclusion It is effective[()treat autoimmune POF mice with
Huluantang combined with progynova
and medroxyprogesterone acetate which may be related with reducing
the Th17 levels and increas-
ing the Treg levels. And it is better than traditional
Chinese medicine group in reducing mice
serum FSH levels.
(Abstract〕 Objective To observe the effects of
Huluantang combined with progynova
and medroxyprogesterone acetate on the expressions of Th17 and
Treg of ovarian in premature
varian failure(POF) mice. Methods The mouse model of autoimmune
POF was established by in-
jetting the zona pellucida 3 peptide fragments (pZP3)
combined with complete Freund adjuvant
(CFA) and incomplete Freund adjuvant (IFA). After immunization,
the mice were randomly divided
into 5 groups as follows: blank group, modeling
group, progynova combined
with medroxyprogesterone group, Huluantang group and
Huluantang combined with progynova
and medroxyprogesterone acetate group. After treatment, follicle
stimulating hormone(FSH) and estra-
diol(EZ) in serum were detected by ELISA, the Th17 and
Treg expression levels in ovarian were
examined by RT-PCR, the ovarian morphology changes were observed
under light microscope. Re-
cults Compared with modeling group, there were therapeutic effects
on treating POF mice in Hulu-
antang combined with progynova and medroxyprogesterone
acetate group, progynova combined
with medroxyprogesterone acetate group and Huluantang group, the
differences were statistically sig-
nificant ( all P <0.01).In terms of二、Iucing serum FSH,
Huluantang combined with progynova
and medroxyprogesterone acetate group and progynova combined
with medroxyprogesterone group
were superior[()Huluantang group (all P<0.05).But in terms of二、
Iucing Th17 levels of ovarian,
Huluantang group was superior[()progynova combined with
medroxyprogesterone group (P<0.05).
Conclusion It is effective[()treat autoimmune POF mice with
Huluantang combined with progynova
and medroxyprogesterone acetate which may be related with reducing
the Th17 levels and increas-
ing the Treg levels. And it is better than traditional
Chinese medicine group in reducing mice
serum FSH levels.
(Abstract〕 Objective To observe the effects of
Huluantang combined with progynova
and medroxyprogesterone acetate on the expressions of Th17 and
Treg of ovarian in premature
varian failure(POF) mice. Methods The mouse model of autoimmune
POF was established by in-
jetting the zona pellucida 3 peptide fragments (pZP3)
combined with complete Freund adjuvant
(CFA) and incomplete Freund adjuvant (IFA). After immunization,
the mice were randomly divided
into 5 groups as follows: blank group, modeling
group, progynova combined
with medroxyprogesterone group, Huluantang group and
Huluantang combined with progynova
and medroxyprogesterone acetate group. After treatment, follicle
stimulating hormone(FSH) and estra-
diol(EZ) in serum were detected by ELISA, the Th17 and
Treg expression levels in ovarian were
examined by RT-PCR, the ovarian morphology changes were observed
under light microscope. Re-
cults Compared with modeling group, there were therapeutic effects
on treating POF mice in Hulu-
antang combined with progynova and medroxyprogesterone
acetate group, progynova combined
with medroxyprogesterone acetate group and Huluantang group, the
differences were statistically sig-
nificant ( all P <0.01).In terms of二、Iucing serum FSH,
Huluantang combined with progynova
and medroxyprogesterone acetate group and progynova combined
with medroxyprogesterone group
were superior[()Huluantang group (all P<0.05).But in terms of二、
Iucing Th17 levels of ovarian,
Huluantang group was superior[()progynova combined with
medroxyprogesterone group (P<0.05).
Conclusion It is effective[()treat autoimmune POF mice with
Huluantang combined with progynova
and medroxyprogesterone acetate which may be related with reducing
the Th17 levels and increas-
ing the Treg levels. And it is better than traditional
Chinese medicine group in reducing mice
serum FSH levels.
(Abstract〕 Objective To observe the effects of
Huluantang combined with progynova
and medroxyprogesterone acetate on the expressions of Th17 and
Treg of ovarian in premature
varian failure(POF) mice. Methods The mouse model of autoimmune
POF was established by in-
jetting the zona pellucida 3 peptide fragments (pZP3)
combined with complete Freund adjuvant
(CFA) and incomplete Freund adjuvant (IFA). After immunization,
the mice were randomly divided
into 5 groups as follows: blank group, modeling
group, progynova combined
with medroxyprogesterone group, Huluantang group and
Huluantang combined with progynova
and medroxyprogesterone acetate group. After treatment, follicle
stimulating hormone(FSH) and estra-
diol(EZ) in serum were detected by ELISA, the Th17 and
Treg expression levels in ovarian were
examined by RT-PCR, the ovarian morphology changes were observed
under light microscope. Re-
cults Compared with modeling group, there were therapeutic effects
on treating POF mice in Hulu-
antang combined with progynova and medroxyprogesterone
acetate group, progynova combined
with medroxyprogesterone acetate group and Huluantang group, the
differences were statistically sig-
nificant ( all P <0.01).In terms of二、Iucing serum FSH,
Huluantang combined with progynova
and medroxyprogesterone acetate group and progynova combined
with medroxyprogesterone group
were superior[()Huluantang group (all P<0.05).But in terms of二、
Iucing Th17 levels of ovarian,
Huluantang group was superior[()progynova combined with
medroxyprogesterone group (P<0.05).
Conclusion It is effective[()treat autoimmune POF mice with
Huluantang combined with progynova
and medroxyprogesterone acetate which may be related with reducing
the Th17 levels and increas-
ing the Treg levels. And it is better than traditional
Chinese medicine group in reducing mice
serum FSH levels.
(Abstract〕 Objective To observe the effects of
Huluantang combined with progynova
and medroxyprogesterone acetate on the expressions of Th17 and
Treg of ovarian in premature
varian failure(POF) mice. Methods The mouse model of autoimmune
POF was established by in-
jetting the zona pellucida 3 peptide fragments (pZP3)
combined with complete Freund adjuvant
(CFA) and incomplete Freund adjuvant (IFA). After immunization,
the mice were randomly divided
into 5 groups as follows: blank group, modeling
group, progynova combined
with medroxyprogesterone group, Huluantang group and
Huluantang combined with progynova
and medroxyprogesterone acetate group. After treatment, follicle
stimulating hormone(FSH) and estra-
diol(EZ) in serum were detected by ELISA, the Th17 and
Treg expression levels in ovarian were
examined by RT-PCR, the ovarian morphology changes were observed
under light microscope. Re-
cults Compared with modeling group, there were therapeutic effects
on treating POF mice in Hulu-
antang combined with progynova and medroxyprogesterone
acetate group, progynova combined
with medroxyprogesterone acetate group and Huluantang group, the
differences were statistically sig-
nificant ( all P <0.01).In terms of二、Iucing serum FSH,
Huluantang combined with progynova
and medroxyprogesterone acetate group and progynova combined
with medroxyprogesterone group
were superior[()Huluantang group (all P<0.05).But in terms of二、
Iucing Th17 levels of ovarian,
Huluantang group was superior[()progynova combined with
medroxyprogesterone group (P<0.05).
Conclusion It is effective[()treat autoimmune POF mice with
Huluantang combined with progynova
and medroxyprogesterone acetate which may be related with reducing
the Th17 levels and increas-
ing the Treg levels. And it is better than traditional
Chinese medicine group in reducing mice
serum FSH levels. |
| Key words: premature ovanan
failure Huluantan} Th17 Trek mouse Cuscu-
Lam. Fructus Mori Rubus chin}ii
premature ovanan
failure Huluantan} Th17 Trek mouse Cuscu-
Lam. Fructus Mori Rubus chin}ii |
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