| 引用本文: |
田莎1，王云丹2，黄立中1*，田雪飞1，许利纯1，伍参荣1，彭吉勇1.阳和汤对裸鼠移植性乳腺癌骨转移模型作用机制的探讨[J].湖南中医药大学学报,2012,32(7):27-30[点击复制] |
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| 阳和汤对裸鼠移植性乳腺癌骨转移模型作用机制的探讨 |
| 田莎1，王云丹2，黄立中1*，田雪飞1，许利纯1，伍参荣1，彭吉勇1 |
| (1.湖南中医药大学中西医结合学院 2.长沙市中医院) |
| 摘要: |
| 目的探讨阳和汤对裸鼠移植性人乳腺癌骨转移模型的作用及其相关调节因子的影响。方法股骨接种人
乳腺癌细胞建立裸鼠乳腺癌骨转移模型。随机分为阳和汤高、低剂量组、唑来磷酸组、模型组、假手术组，动态观察各组
裸鼠骨转移癌的生长情况， 测量骨转移癌体积， 绘制生长曲线； 给药20 d 后处死动物， 测取瘤质量， 计算抑瘤率；
ELISA 法检测裸鼠血清中相关调节因子PTHrP、RANKL、OPG 含量的变化。结果1.阳和汤高、低剂量组、唑来磷酸组的
骨转移癌灶体积均小于模型组，差异有统计学意义（P＜0.05）；2.阳和汤高、低剂量组、唑来磷酸组PTHrP 和RANKL 含
量明显低于模型组，差异有统计学意义（P＜0.05），而OPG 含量水平增加，RANKL/OPG 的比率下降，差异有统计学意义
（P＜0.05）。结论1.阳和汤有抗移植性人乳腺癌骨转移作用；2.阳和汤下调PTHrP、RANKL 的含量、增加OPG 的含量，
抑制RANKL/OPG 比率可能是其抑制乳腺癌骨转移的作用机制之一。 |
| 关键词: 乳腺癌 骨转移 阳和汤 甲状旁腺激素相关蛋白 NF-KB 配体的受体 骨保护素 裸鼠 熟地黄 鹿角胶 |
| DOI： |
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| 基金项目:湖南省高校创新平台开放基金项目（09k058）；高等学校博士学科点专项科研基金资助项目（20114323110006）；湖南省教育厅资助项目（07C488）。 |
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| Effect mechanism of Yanghe decoction on bone metastasisof human breast cancer xenografts in nude mice |
| TIAN Sha1， WANG Yun-dan2， HUANG Li-zhong1， TIAN xue-fei1， XU Li-chun,WU Can-rong1， PENG Ji-yong1 |
| (1. College of Integrated Chinese and Western medicine，TCM University of Hunan 2. Chinese Medicine hospital of Changsha) |
| Abstract: |
| Objective To study the effects of Yanghe decoction on bone metastasis of human
breast cancer xenografts in nude mice and the impacts in relevant regulatory factors. Methods
The model of bone metastasis of human breast cancer xenografts in nude mice was established by
inoculating human breast cancer cells in femur. These mice were randomly divided into 5 groups
as follows: Yanghe decoction high -dose group and low -dose group， zoledronic
acid group， model group and sham-operated group. Bone metastasis tumors of different groups were
observed dynamically, the volume and the growth curves of the bone metastasis tumors
were measured during treatment phase. After treatment for 20 d, tumor weights were measured
and the inhibition rates were calculated. The levels of associated regulatory factors of PTHrP，RANKL
and OPG in nude mice serum were examined by ELISA enzyme-linked assay. Results The
focal tumors in the Yanghe decoction high-dose group and low-dose group，zoledronic acid groupwere smaller than those in model group, and the differences between them were statistically significant(
all P<0.01). Compared with model group，the content of PTHrP and RANKL were significantly
decreased in the Yanghe decoction high-dose group and low-dose group， zoledronic acid group
(all P<0.05), while the OPG levels were increased，and the ratios of RANKL/OPG were decreased
significantly (all P <0.05). Conclusion Yanghe decoction has an effects on inhibiting the
bone metastasis of human breast cancer, the mechanism may be related to the down-regulation of
PTHrP and RANKL levels, up-regulation of OPG level and thus decreasement of RANKL/OPG ratio. |
| Key words: breast cancer bone metastasis Yanghe decoction PTHrP RANKL OPG nude mouse chinese foxglove deerhorn glue |
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