| 引用本文: |
谢谊； 易艳； 刘阳； 彭一波； 王实强；.柱前衍生HPLC测定阿胶中17种水解氨基酸含量[J].湖南中医药大学学报,2012,32(5):46-49[点击复制] |
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| 柱前衍生HPLC测定阿胶中17种水解氨基酸含量 |
| 谢谊；易艳；刘阳；彭一波；王实强； |
| (湖南省中医药研究院中药研究所; 湖南省娄底市中心医院; 湖南中医药大学) |
| 摘要: |
| 目的:建立柱前衍生高效液相色谱法(HPLC)同时测定阿胶中17种水解氨基酸含量的方法。方法用6 mol/L盐酸溶液水解阿胶中的氨基酸,以异硫氰酸苯酯为柱前衍生试剂;用Hypersil BDS C18柱(200 mm×4.6 mm,5μm),梯度洗脱,流速为1.0 mL/min,检测波长为254 nm,柱温为30℃。结果 17种氨基酸在60 min内均可得到很好的分离,回收率为97.4%～100.1%,RSD为1.6%～3.4%。结论建立的方法可靠,可用于阿胶中17种水解氨基酸的含量测定。 |
| 关键词: 阿胶 氨基酸 酸水解 柱前衍生高效液相色谱法 |
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| Determination of 17 kinds of hydrolyzed amino acids content in donkey-hide glue by HPLC with pre-colimn derivatization |
| XIE Yi,YI Yang,LIU Yang,PENG Yi-bo,WANG Shi-qiang |
| (Institute of Chinese Materia Medica,TCM Academy of Hunan) |
| Abstract: |
| Objective To determine the contents of 17 kinds of hydrolyzed amino acids in donkey-hide glue by HPLC with pre一〔column derivatization at the same time. Methods To extract amino acids by the method of hydrolysis in 6 mol/L HCl and use phenylisothiocyanate (PITC) as the derivating agent. The Hypersil BDS C,8 column (200 mmx4.6 mm, 5μm) was used. Under the condition of gradient elution, the flow rate was 1.0 mL/min, the UV detection wavelength was 254 nm, and the column temperature was 30 0C. Results 17 kinds of amino acids can be separated well in 60 min. The average recoveries were 97.4%一100.1% and RSD were 1.6%}3.4%. Conclusion The method is reliable for the determination of 17 kinds of amino acids in donkey-hide glue. |
| Key words: donkey-hide glue 17 kinds of amino acids acid hydrolysis HPLC with pre-column derivatization |
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