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李卓； 邓桂明； 郭昱； 王文娜； 张鹤鸣；.复方三七片质量标准研究[J].湖南中医药大学学报,2011,31(11):34-37[点击复制] |
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| 复方三七片质量标准研究 |
| 李卓；邓桂明；郭昱；王文娜；张鹤鸣； |
| ((1.华南师范大学中药及创新药物研究所、国家中医药昔理局中医药与光子技术三级实验室，广东广州51063122.湖南中医药大学第一附属医院药剂科，湖南长沙410007) |
| 摘要: |
| 目的研究成方制剂复方三七片的质量标准。方法采用TLC对白芷、三七进行定性鉴别;采用HPLC进行主成分含量测定,色谱柱为Aglient ZORBAX C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速为1.0 mL/min,检测波长为203 nm。结果 TLC可鉴别三七和白芷,HPLC测定人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1,三者分别在0.048～0.384 mg、0.052 5～0.420 mg、0.011 8～0.094 4 mg范围内线性、精密度、稳定性均良好,阴性无干扰,平均加样回收率分别为100.91%(RSD=1.04%)、100.36%(RSD=1.04%)、100.29%(RSD=0.99%)。结论所建立的质量标准简便可行、重复性好,可以用来评价复方三七片的质量。 |
| 关键词: 复方三七片 质量标准 HPLC TLC 人参皂苷Rg1 人参皂苷Rb1 三七皂苷R1 |
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| Studies on quality standard of compound pseudo}inseng tablets |
| LI Zhuo1,DENG Gui-ming1,2,GUO Yu1,WANG Wen-na1,ZHANG He-ming1 |
| (1.Tertiary Laboratory of Chinese Medicine & Photonics,State Administration of Chinese Medicine,South China Normal University,Guangzhou 5106312;2.First Affiliated Hospital,TCM University of Hunan,Changsha,Hunan 410007,China) |
| Abstract: |
| Objective To establish a quality standard for compound Pseudo-Ginseng tablets Methods Angelica dahurica and Pseudo-Ginseng were identified by thin layer chromatography. The content of Ginsenoside Rg,, Ginsenoside Rb, and Notoginsenoside R, were determined by High Performance Liquid Chromatography. Chromatographic conditions: Aglient ZORBAX C,8 column (150 mmx4.6 mm, 5 N.,m) was used as the stationary phase, a mixture of acetonitrile and water as the mobile phase was adopted. The flow rate was 1.0 mL/min, and the detection wavelength at 203 nm. Results The qualitative identification with TLC were specific. The linear response range was0.048一 0.384 mg/mL for Ginsenoside Rg,, 0.0525一 0.420 mg/mL for Ginsenoside Rb,, and 0.0118-0.0944mm for Notogin senoside R,. The average二〔eoveries of these compounds were 100.91%(RSD=1.04%), 100.36%(RSD=1.04%), 100.29%(RSD=0.99%), respectively. Conclusion This method is simple, accurate and reproducible for quality control of Compound Pseudo-Ginseng Tablet. |
| Key words: compound Pseudo-Ginseng tablets quality control HPLC TLC ginsenoside Rg, ginsenoside Rb, notoginsenoside R, |
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