| 引用本文: |
谭琦 黄政德 王立凤.大鼠骨髓间充质干细胞分离和培养方法的研究[J].湖南中医药大学学报,2011,31(7):74-76[点击复制] |
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| 大鼠骨髓间充质干细胞分离和培养方法的研究 |
| 谭琦黄政德王立凤 |
| (}1湖南中医药大学2008级博士研究生班，湖南长沙410208;2湖南中医药大学，湖南长沙410208
3湖南中药大学第二附属医院，湖南长沙410006)) |
| 摘要: |
| [摘要〕目的研究在体外培养和纯化SD大鼠骨髓间充质干细胞(BMSCs)的方法〔方法采用改良全骨髓贴壁
方法，分离和纯化3-4周的大鼠BMSCs，镜卜连续观察细胞的形态变化、流式细胞仪鉴定其表面抗原CD45和CD29
均表达情况〔结果原代培养的细胞呈圆形和梭形等，24 h后大部分细胞均贴壁〔8-10 d可达800Ic-90%融合，纯化
青传代周期为6-8 d〔流式细胞术鉴定表明C D45阴性，CD29阳性〔结论改良全骨髓贴壁法可有效分离培养大鼠
}'J骨髓间充质干细胞，是一种比较理想的分离培养方法、 |
| 关键词: 骨髓间充质干细胞
细胞培养
大鼠 |
| DOI： |
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| 基金项目:国家自然科学基金项日(30973750);湖南省教育厅重点项日(09A071);2010午湖南省研究生科研创新项日((CX2010B341) |
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| Studyof rat boneon isolation and culturemarrow mesenchymal stem cells |
| TAN Qi, HUANG Zheng-de, WANG Li-feng |
| ((TCM University of Hunan, Changsha, Hunan, 410208, China)) |
| Abstract: |
| (Abstract〕 Objective To explore a puerification and culture method of bone marrow mesenchymal
stem cells (BMSCs) in rat in vitro. Methods BMSCs from bone marrow of 3}4 week old rats were sepa-
rated and purified by a improved method of whole bone marrow adherent.
The morphological changes of BMSCs were observed continuously under the microscope. The ex-
pression of surface antigens CD45 and CD29 were analyzed by flow cytometry. Results Cultured
primary BMSCs were circular or spindle -shaped and the most BMSCs were adherent within
24 hours, and 80%}90% BMSCs were in fusion within 8一10 days. The Transfer
of generation cycle was 6}8 days after purification. The Flow cytometry identification showed that
CD29 was positive and CD45 negative. Conclusion The improved method of whole bone marrow
adherent. can successfully isolate and culture BMSCs in rats, which is proved[()be an opti-
mal methods for cell preparation and culture. |
| Key words: [)()ne
marroh
mesenchymal stem cells(BMSCs) cell culture rats |
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