| 引用本文: |
邓颖’，需鹏2*，朱诗塔2，李新中’理，胡随瑜’，张春虎.大黄不同炮制品的HPLC指纹图谱比较研究[J].湖南中医药大学学报,2011,31(3):39-41[点击复制] |
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| 大黄不同炮制品的HPLC指纹图谱比较研究 |
| 邓颖’，需鹏2*，朱诗塔2，李新中’理，胡随瑜’，张春虎 |
| (中南大学湘雅医院中西医结合研究所，国家中医药管理局肝脏象重点研究室，中南大学湘雅医院药剂科) |
| 摘要: |
| 〔摘要〕目的对大黄不同炮制品进行HPLC指纹图谱的比较研究，为大黄不同炮制品的鉴别及炮制的作用机
制提供依据方法采用HPLC色谱法，色谱柱Diamonsil C,H(4.6 mmx250 mm,5 }e,m)，流动相:甲醇-0.050Ic磷酸
梯度洗脱，检测波长280 nm，柱温35℃，流速1.0 mL/min，以指纹图谱软件计算相似度，并进行图谱比较〔结果
大黄不同炮制品的HPLC指纹图谱共有峰特征明显，不同大黄炮制品间的成分差异显著〔结论木法简便、快捷、重
复性好.利用HPLC指纹图谱分析方法.可较全而的反映大黄不同炮制品的差异、 |
| 关键词: 大黄 炮制 HPLC指纹图谱 |
| DOI： |
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| 基金项目:国家自然科学基金青午基金(30801506);湖南省卫生厅课题(B20090013) |
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| Comparison researchon different products ofof HPLC fingerprintRadix et Rhizoma Rhei |
| DENG Ying, LEI Peng, ZHU Shi-ta, LI Xin-zhong, HU Sui-yu, ZHANG Chun-hu |
| (Institute of Integrated Chinese and Western Medicine, I}ey Laboratory of TCM liver of SATCM
,Xiangya Hospital, Central South University) |
| Abstract: |
| Rhei
‘eSSed
[Abstract] Objective To
for different processed
set up the HPLC fingerprint chromatogram of Radix et Rhizoma
products and offer evidence for identifying different pro-
methods. Method The HPLC fingerprint
method was used on
a Diamonsil C,R column
(4.6 mm x250 mm , 5 p.,m) with
mobile
phase in gradient elution
1 mlJmin. The detection
and the flowing speed
composed of
wavelength
methanol and
0.05% phosphoric acid at a flow
the column temperature was 35
The characteristic of common peaks
Rhizoma Rhei is distinct, and there
rate:
℃，
、、as
、、as
1.0
for fingerprints of different samples of
are differences among various processed
Rhei. Conclusion The method is convenient, rapid and repeat well,which
fy different samples of processed Radix et Rhizoma Rhei.
can
mL/min.
processed
Radix et
he used
280 nm,
Results
Radix et
Rhizoma
[()7(leTltl- |
| Key words: Radix et Rhizoma Rhei processing HPLC fingerprint |
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