| 引用本文: |
覃妮,陆世银,罗文婷,陈家豪,龙嘉怡,黄仁彬.玉郎伞多糖对S180荷瘤小鼠氧化应激、免疫调节及血管生成的影响[J].湖南中医药大学学报,2022,42(11):1849-1854[点击复制] |
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| 玉郎伞多糖对S180荷瘤小鼠氧化应激、免疫调节及血管生成的影响 |
| 覃妮,陆世银,罗文婷,陈家豪,龙嘉怡,黄仁彬 |
| (柳州市中医医院(柳州市壮医医院)药学部, 广西 柳州 545026;广西医科大学药学院, 广西 南宁 530021) |
| 摘要: |
| 目的 探讨玉郎伞多糖（Yulangsan polysaccharides, YLSPS）对S180荷瘤小鼠的体内抗肿瘤作用及其机制。方法 以昆明小鼠建立S180皮下移植瘤模型，随机分为模型组、环磷酰胺（cyclophosphamide, CTX 20 mg·kg-1·d-1）组、YLSPS低，中，高剂量（150、300、600 mg·kg-1·d-1）组，连续给药8 d后处死小鼠，检测小鼠瘤体质量、抑瘤率及其脏器指数；通过HE染色观察小鼠移植瘤细胞的形态学特征；测定血清中超氧化物歧化酶（superoxide dismutase, SOD）、谷胱甘肽过氧化物（glutathione peroxidase, GSH-PX）活性，丙二醛（malondialdehyde, MDA）及过氧化氢酶（catalase, CAT）的含量；采用ELISA法测定小鼠血清中血管内皮生长因子（vascular endothelial growth factor, VEGF）的含量；免疫组织化学法检测药物对微血管密度（microvascular density, MVD）表达的影响。结果 与模型组比较，YLSPS （300、600 mg·kg-1·d-1）可显著抑制S180荷瘤小鼠的肿瘤生长，提高小鼠脾脏指数和胸腺指数（P＜0.05或P＜0.001）；移植瘤HE染色表明，相较于模型组，经YLSPS干预后肿瘤细胞异型性降低，病理性核分裂减少，可见散在及融合成片状的坏死灶；血清生化指标显示YLSPS （300、600 mg·kg-1·d-1）能够明显升高血清GSH-Px、CAT和SOD水平，降低血清MDA及VEGF水平（P＜0.05或P＜0.001）；ELISA法与免疫组织化学法表明YLSPS （300、600 mg·kg-1·d-1）可显著抑制VEGF、MVD 的表达（P＜0.05或P＜0.001）。结论 YLSPS可抑制荷瘤小鼠肿瘤的生长，其机制可能与增强小鼠免疫功能、抗氧化作用及抑制S180肿瘤组织中VEGF与MVD 的表达有关。 |
| 关键词: 玉郎伞多糖 S180荷瘤小鼠 微血管密度 血管内皮生长因子 抗氧化 |
| DOI：10.3969/j.issn.1674-070X.2022.11.013 |
| 投稿时间：2022-03-31 |
| 基金项目:国家工程中心中药固体制剂制造技术国家工程研究中心——桂林医学院联合开发基金资助项目（JG201507A1）；广西自然科学基金项目（2012GXNSFBA053094）。 |
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| Effects of Yulangsan polysaccharide on oxidative stress, immune regulation and angiogenesis in S180 tumor-bearing mice |
| QIN Ni,LU Shiyin,LUO Wenting,CHEN Jiahao,LONG Jiayi,HUANG Renbin |
| (Department of Pharmacy, Liuzhou Hospital of Chinese Medicine (Liuzhou Hospital of Zhuang Medicine), Liuzhou, Guangxi 545026, China;School of Pharmacy, Guangxi Medical University, Nanning, Guangxi 530021, China) |
| Abstract: |
| Objective To investigate in vivo anti-tumor effects and mechanism of Yulangsan polysaccharide (YLSPS) on S180 tumor-bearing mice. Methods The S180 subcutaneous transplanted tumor model was established by Kunming mice. All mice were randomly divided into model group, cyclophosphamide (CTX 20 mg·kg-1·d-1) group, YLSPS low, medium and high dosage (150, 300 and 600 mg·kg-1·d-1) groups, and they were sacrificed after 8 d of continuous administration. The tumor weights, tumor inhibition rates and organ indexes were measured; morphological characteristics of transplanted tumor cells were identified by HE staining; the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), the activity of malondialdehyde (MDA) and catalase (CAT) in serum were checked; the content of vascular endothelial growth factor (VEGF) in mice serum was determined by ELISA; drug effects on microvascular density (MVD) expression were detected by immunohistochemistry. Results Compared with the model group, YLSPS （300, 600 mg·kg-1·d-1） significantly inhibited the tumor growth of S180 tumor-bearing mice and increased the spleen index and thymus index (P＜0.05 or P＜0.001). Compared with the model group, HE staining indicated that tumor cell atypia and pathologic karyokinesis decreased after YLSPS intervention; diffused and flaked necrotic foci were observed. Serum biochemical index showed that YLSPS (300, 600 mg·kg-1·d-1) could significantly increase serum GSH-Px, CAT and SOD levels and decrease serum MDA and VEGF levels (P＜0.05 or P＜0.001); ELISA and immunohistochemistry indicated that YLSPS (300, 600 mg·kg-1·d-1) could significantly inhibit the expression of VEGF and MVD (P＜0.05 or P＜0.001). Conclusion YLSPS could inhibit the tumor growth in S180 tumor-bearing mice, and the mechanism may be related to enhancing immune function and anti-oxidation, and inhibiting the expression of VEGF and MVD in S180 tumor tissues. |
| Key words: Yulangsan polysaccharide S180-bearing mice microvascular density vascular endothelial growth factor antioxidation |
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