| 引用本文: |
李跃兵,王焕梅,孙柳青,陈佳怡,侯培媚,李筝,佘艳,彭艳.电针调节功能性消化不良大鼠胃肠动力障碍及对胃窦组织铁死亡相关因子的影响[J].湖南中医药大学学报,2022,42(8):1329-1334[点击复制] |
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| 电针调节功能性消化不良大鼠胃肠动力障碍及对胃窦组织铁死亡相关因子的影响 |
| 李跃兵,王焕梅,孙柳青,陈佳怡,侯培媚,李筝,佘艳,彭艳 |
| (湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208;武汉市第九医院康复科, 湖北 武汉 430010) |
| 摘要: |
| 目的 观察电针调节功能性消化不良(functional dyspepsia,FD）大鼠胃肠动力障碍及对胃窦组织铁死亡相关因子的影响。方法 将40只SD大鼠随机分为空白组、模型组、电针组、西药组，每组10只。除空白组外，其余各组采用多因素干预法制造FD大鼠模型。造模成功后，模型组不采用干预措施，电针组采用电针足三里穴治疗21 d，西药组采用多潘立酮灌胃21 d。干预结束后，观察大鼠胃排空率、小肠推进率、胃窦组织亚铁离子（Fe2+）含量、还原型谷胱甘肽（glutathione,GSH）浓度、丙二醛（malondialdehyde,MDA）浓度、细胞脂质活性氧族簇（reactive oxygen species,ROS）含量，谷胱甘肽过氧化物酶4（glutathione peroxidase 4,GPX4）mRNA、胱氨酸/谷氨酸反向转运蛋白（cystine/glutamate reverse transporter,system Xc-）mRNA、GPX4蛋白、system Xc-蛋白表达。结果 与空白组相比，模型组大鼠胃排空率、小肠推进率、GSH浓度、GPX4mRNA、system Xc-mRNA、GPX4蛋白、system Xc-蛋白表达水平均降低，胃窦组织Fe2+含量、MDA浓度、细胞脂质ROS含量均升高（P<0.05）；与模型组相比，电针组、西药组大鼠胃排空率、小肠推进率、GSH浓度、GPX4mRNA、system Xc-mRNA、GPX4蛋白、system Xc-蛋白表达水平均升高，胃窦组织Fe2+含量、MDA浓度、细胞脂质ROS含量均降低（P<0.05）；与西药组相比，电针组大鼠胃排空率、小肠推进率、胃窦组织GSH浓度、GPX4mRNA、system Xc-mRNA、GPX4蛋白、system Xc-蛋白表达水平升高，胃窦组织Fe2+含量、MDA浓度、细胞脂质ROS含量降低(P<0.05)。相关性分析结果显示：各组大鼠胃排空率、小肠推进率与胃窦组织Fe2+含量、MDA浓度、细胞脂质ROS含量成正相关（r=0.50，P<0.05），与GSH、GPX4mRNA、system Xc-mRNA、GPX4蛋白、system Xc-蛋白呈负相关（r=-0.51，P<0.05）。结论 电针对FD大鼠胃肠动力障碍有良性调节作用，其作用机制可能是通过抑制胃窦组织细胞铁死亡来实现的。 |
| 关键词: 电针 功能性消化不良 胃肠动力障碍 胃窦组织 铁死亡 胃排空率 小肠推进率 MDA浓度 ROS含量 |
| DOI：10.3969/j.issn.1674-070X.2022.08.015 |
| 投稿时间：2022-01-05 |
| 基金项目:国家自然科学基金项目（81774431）；武汉市卫健委科研项目（WZ21Q21）；湖南中医药大学中西医结合国内一流学科建设开放基金项目（2020ZXY16)。 |
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| Effect of electroacupuncture on regulating gastrointestinal motility disorder in functional dyspepsia and iron death related factors in gastric antrum in rats with functional dyspepsia |
| LI Yuebing,WANG Huanmei,SUN Liuqing,CHEN Jiayi,HOU Peimei,LI Zheng,SHE Yan,PENG Yan |
| (College of Acupuncture, Massage and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Rehabilitation Department of Wuhan Ninth Hospital, Wuhan, Hubei 430010, China) |
| Abstract: |
| Objective To observe the effect of electroacupuncture on regulating gastrointestinal motility disorder and iron death related factors in gastric antrum in rats with functional dyspepsia (FD). Methods A total of 40 SD rats were randomly divided into blank group, model group, electroacupuncture group and western medicine group, with 10 rats in each group. Except for the blank group, the other groups used multifactor intervention to create the rat model of FD. After successful modeling, the model group did not use intervention measures. The electroacupuncture group was treated with electroacupuncture at Zusanli ("ST36") for 21 d, and the western medicine group was treated with domperidone by gavage for 21 d. After the intervention, the gastric emptying rate, intestinal propulsion rate, ferrous ion (Fe2+) content in gastric antrum, reduced glutathione (GSH) concentration, malondialdehyde (MDA) concentration, cellular lipid reactive oxygen species (ROS) content, glutathione peroxidase4 (GPX4) mRNA, cystine/glutamate reverse transporter (system Xc-) mRNA, GPX4 protein, system XC protein expression were observed. Results Compared with the blank group, the gastric emptying rate, intestinal propulsion rate, GSH concentration, GPX4mRNA, system Xc-mRNA, GPX4 protein, system XC-protein expression levels decreased in the model group, while Fe2+ content, MDA concentration and cellular lipid ROS content increased in the gastric antrum tissue (P<0.05); compared with the model group, the gastric emptying rate, intestinal propulsion rate, GSH concentration, GPX4mRNA, system Xc-mRNA, GPX4 protein, system Xc-protein expression levels increased in the electroacupuncture group and western medicine group, while Fe2+ content, MDA concentration and cellular lipid ROS content decreased in the gastric antrum (P<0.05); compared with the western medicine group, the gastric emptying rate, intestinal propulsion rate, GSH concentration, GPX4mRNA, system Xc-mRNA, GPX4 protein, system Xc-protein expression levels in gastric antrum tissue of electroacupuncture group increased, while Fe2+ content, MDA concentration and cellular lipid ROS content in gastric antrum tissue decreased (P<0.05). The results of correlation analysis showed that the gastric emptying rate and intestinal propulsion rate of rats in each group were positively correlated with Fe2+ content, MDA concentration and cellular lipid ROS content in gastric antrum (r=0.50, P<0.05), and negatively correlated with GSH, GPX4mRNA, system Xc-mRNA, GPX4 protein and system Xc-protein (r=-0.51, P<0.05). Conclusion Electroacupuncture has a benign regulatory effect on gastrointestinal motility disorder in FD rats, and its mechanism may be achieved by inhibiting iron death in gastric antrum tissue. |
| Key words: electroacupuncture functional dyspepsia gastrointestinal motility disorder gastric antrum ferroptosis gastric emptying rate intestinal propulsion rate MDA concentration ROS content |
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