| 引用本文: |
吴源陶,邹译娴,张春虎,王理槐.菝葜皂苷元对结直肠癌细胞HT-29凋亡和自噬的影响[J].湖南中医药大学学报,2021,41(11):1645-1649[点击复制] |
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| 菝葜皂苷元对结直肠癌细胞HT-29凋亡和自噬的影响 |
| 吴源陶,邹译娴,张春虎,王理槐 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;中南大学湘雅医院中西医结合科, 湖南 长沙 410008) |
| 摘要: |
| 目的 探讨菝葜皂苷元（sarsasapogenin，SAR）对人结直肠癌细胞HT-29增殖、凋亡和自噬的影响。方法 将HT-29细胞分为对照组（DMEM培养液）和SAR组（DMEM培养液+DMSO+10、20、30、40、50、60 mg/L SAR溶液）；分别采用MTT法、流式细胞术、TUNEL染色及MDC染色检测细胞的增殖、凋亡及自噬的变化情况；用Western blot法检测细胞中Caspase-3、Caspase-9、Beclin-1及LC3B蛋白表达水平。结果 经SAR处理后，HT-29细胞的增殖能力明显降低（P<0.05），凋亡和自噬水平明显增加（P<0.05）；与对照组相比，SAR组细胞Caspase-3、Caspase-9、Beclin-1及LC3B蛋白表达水平显著上调（P<0.05）。结论 SAR能够抑制结直肠癌细胞增殖，诱导细胞凋亡和细胞自噬的发生，其机制可能与上调Caspase-3、Caspase-9、Beclin-1及LC3B蛋白表达水平有关。 |
| 关键词: 菝葜皂苷元 结肠癌 大肠癌 细胞增殖 细胞凋亡 细胞自噬 |
| DOI：10.3969/j.issn.1674-070X.2021.11.001 |
| 投稿时间：2020-12-24 |
| 基金项目:国家自然科学基金项目（81673951）；湖南省卫健委资助项目（C2019092）；湖南省教育厅资助项目（13C054）。 |
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| Effect of Sarsasapogenin on Apoptosis and Autophagy of Colorectal Cancer Lines HT-29 |
| WU Yuantao,ZOU Yixian,ZHANG Chunhu,WANG Lihuai |
| (The First Affiliated Hospital of Hunan University of Chines Medicine, Changsha, Hunan 410007, China;Xiangya Hospital, Central South University, Changsha, Hunan 410008, China) |
| Abstract: |
| Objective To investigate the effect of sarsasapogenin (SAR) on the proliferation, apoptosis, and autophagy of human colorectal cancer cell HT-29. Methods HT-29 cells were divided into control group (DMEM medium) and SAR group (DMEM medium + DMSO + 10, 20, 30, 40, 50, 60 mg/L SAR solution). MTT, flow cytometry, TUNEL staining, and MDC staining were used to detect the changes of cell proliferation, apoptosis and autophagy; Western blot method was used to detect the expression levels of Caspase-3, Caspase-9, Beclin-1 and LC3B protein. Results After treatment with SAR, the proliferation ability of HT-29 cells was significantly reduced (P<0.05), and the level of apoptosis and autophagy increased significantly (P<0.05). Compared with the control group, the expression levels of Caspase-3, Caspase-9, Beclin-1 and LC3B protein in SAR group were significantly increased (P<0.05). Conclusion SAR can inhibit the proliferation of colorectal cancer cells, induce apoptosis and autophagy. The mechanism may be related to the up-regulation of Caspase-3, Caspase-9, Beclin-1 and LC3B protein expression levels. |
| Key words: sarsasapogenin colon cancer colorectal cancer cell proliferation apoptosis autophagy |
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