| 引用本文: |
蒋冰,王妙华,谌爱华,王韧,李文姣,陈思阳,邓凯文,贺福元.针刺心经神门与少海对补阳还五汤谱动学研究[J].湖南中医药大学学报,2020,40(6):732-738[点击复制] |
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| 针刺心经神门与少海对补阳还五汤谱动学研究 |
| 蒋冰,王妙华,谌爱华,王韧,李文姣,陈思阳,邓凯文,贺福元 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学药学院, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;中药成药性与制剂制备湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学 中医药超分子机理与数理特征化实验室, 湖南 长沙 410208;湖南中医药大学药学院, 湖南 长沙 410208;中药成药性与制剂制备湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学 中医药超分子机理与数理特征化实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 通过针刺大鼠心经神门与少海两穴，观察其对补阳还五汤谱动学的影响，验证针药并用可改变中药代谢的推断；方法 针刺大鼠心经的神门与少海；补阳还五汤血样指纹图谱采用高效液相色谱法测定，其条件为：（1）色谱柱：Ultimate AQ-C18，4.6 mm×250 mm，5 μm；（2）检测波长：264 nm；（3）流动相：甲醇∶水采用梯度洗脱，流速为1 mL·min-1；（4）温度：35℃。谱动学采用指纹图谱与药物动力学相结合的总量统计矩评价方法。结果 针刺组补阳还五汤谱动学参数：总量半衰期为7.726 h，经0~24.51 h总量的95%成分被排出体外，其指纹图谱中95%的成分的保留时间在0~92.37 min之内；而对照组补阳还五汤谱动学参数：总量半衰期为10.09 h，经0.45~28.67 h总量的95%成分被排出体外，其指纹图谱95%的成分在0~95.59 min之内。结果显示针刺大鼠心经神门与少海能减少补阳还五汤谱动学的半衰期，增快药物代谢。结论 针刺经络腧穴能调整脏腑功能，反作用于中药代谢，其谱动学会产生明显变化，对应药效也会产生变化，针药并用可改变中药量-时-效作用规律。 |
| 关键词: 针药并用 针刺 神门 少海 补阳还五汤 经络脏象 代谢指纹图谱 谱动学 |
| DOI：10.3969/j.issn.1674-070X.2020.06.018 |
| 投稿时间：2019-09-16 |
| 基金项目:国家自然科学基金项目（81573691,81874507）；湖南省自然科学基金项目（2017JJ3236）；湖南省教育厅基金项目（17B200）；湖南省中医学双一流学科；中药成药性与制剂制备湖南省重点实验室。 |
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| Spectrokinetic Study on Buyang Huanwu Decoction by Acupuncture at Shenmen (TF4) and Shaohai (HT3) in Heart Channel |
| JIANG Bing,WANG Miaohua,CHEN Aihua,WANG Ren,LI Wenjiao,CHEN Siyang,DENG Kaiwen,HE Fuyuan |
| (The First Affinity Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;The First Affinity Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affinity Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan Provincial Key Laboratory of Drugability and Preparation Modification of TCM, Changsha, Hunan 410208, China;Supramolecular Mechanism and Mathematic-Physics Characterization Laboratory for Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory of Drugability and Preparation Modification of TCM, Changsha, Hunan 410208, China;Supramolecular Mechanism and Mathematic-Physics Characterization Laboratory for Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects on the spectrokinetic of Buyang Huanwu Decoction by acupuncturing at Shenmen (TF4) and Shaohai (HT3) in heart channel of rats, to verify the inference of the combined use of acupuncture and medicine can change the metabolism of Chinese materia medica. Methods Shenmen (TF4) and Shaohai (HT3) in heart channel of rats were acupunctured. The metabolic chromatographic fingerprint of the Buyang Huanwu Decoction was determined by HPLC, under conditions of (1) Ultimate AQ-C18 column: 4.6 mm×250 mm, 5 μm; (2) the detection of wavelengths: 264 nm.; (3) the mobile phase: gradient elution by mixture of methyl cyanides and water; the mobile velocity was 1 mL·min-1; (4) the temperature: 35 ℃. Spectrokinetic used a method of total statistical moment evaluation that combines fingerprints and pharmacokinetics. Results Spectrokinetic parameters in the acupuncture group were: the half-life of the total amount was 7.726 h, and 95% of the total amount was excluded after 0~24.51 h. The retention time of 95% of the components in the fingerprint was 0~92.37 min. In the control group, the half-life of the total amount was 10.09 h, and 95% of the total amount was excluded after 0.45~28.67 h, and 95% of the fingerprint was within 0~95.59 min. The results showed that acupuncture at Shenmen (TF4) and Shaohai (HT3) in heart channel of rats could reduce the half-life of the spectrokinetic of Buyang Huanwu Decoction and increase the drug metabolism. Conclusion Acupuncture at acupoints in meridians can regulate the function of zang-fu organs, which can adversely affect the metabolism of Chinese materia medica. Its spectrokinetic changes significantly, and the corresponding drug effects also change. The combined use of acupuncture and medicine can change the amount-time-effect law of Chinese materia medica. |
| Key words: acupuncture combined with medicine acupuncture Shenmen (TF4) Shaohai (HT3) Buyang Huanwu Decoction meridian and visceral manifestations metabolic fingerprint spectrokinetic |
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