| 引用本文: |
李蒙,文颖娟,杨俊超,王江,柏鲁宁.葛根及其配伍对重症肌无力大鼠骨骼肌线粒体结构的影响[J].湖南中医药大学学报,2020,40(2):144-149[点击复制] |
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| 葛根及其配伍对重症肌无力大鼠骨骼肌线粒体结构的影响 |
| 李蒙,文颖娟,杨俊超,王江,柏鲁宁 |
| (陕西中医药大学, 陕西 咸阳 712046;陕西中医药大学附属医院, 陕西 咸阳 712000) |
| 摘要: |
| 目的 探讨葛根及其配伍对重症肌无力大鼠骨骼肌线粒体结构的作用机制。方法 （1）造模：给模型大鼠以腹部、足垫、背部皮下注射200 μL（含R 97-116：100 μg）免疫乳剂进行造模，30 d和45 d后，分别以同样方法对造模大鼠强化接种，以大鼠Lennon肌力评分 ≥ 1级为模型鼠。（2）分组与处理：除空白组外，造模成功鼠随机分为模型组、强的松组、葛根组、葛芪组、葛参组、葛妙组、葛芪参妙组，每组20只。造模成功2周后，给予空白组、模型组蒸馏水灌胃，其他组予相应药物灌胃，连续4周。（3）标本检测：透射电镜观察骨骼肌线粒体超微结构；免疫组化法检测FGF、NGF、IGFs表达。结果 与模型组比较，各组的线粒体形态均有恢复，其中葛芪组和葛芪参妙组治疗后整体形态恢复较好；与模型组比较，各治疗组FGF、NGF、IGFs的阳性表达率都有所升高，差异有统计学意义（P<0.01）。结论 葛根及其配伍可能通过改善肌肉生成调控因子的阳性表达促进线粒体结构的恢复，达到治疗重症肌无力的目的。 |
| 关键词: 重症肌无力 葛根配伍 骨骼肌线粒体超微结构 肌肉生成调控因子 |
| DOI：10.3969/j.issn.1674-070X.2020.02.005 |
| 投稿时间：2019-05-14 |
| 基金项目:国家自然科学基金资助项目（81202642）；陕西省科学技术厅资助项目（2017SF-323）；第四批全国中医（临床、基础）优秀人才研修项目（国中医药人教发〔2017〕24号）。 |
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| Effects of Radix Puerariae and its Compatibility on Mitochondrial Structure of Skeletal Muscle in Myasthenia Gravis Rats |
| LI Meng,WEN Yingjuan,YANG Junchao,WANG Jiang,BAI Luning |
| (Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, China;The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712000, China) |
| Abstract: |
| Objective To explore the action mechanism of Radix Puerariae and its compatibility on the mitochondrial structure of skeletal muscle in myasthenia gravis rats. Methods (1)modeling:The model rats were established by injecting 200 μL (including r 97-116:100 μg) immune emulsion into abdomen, foot pad and back subcutaneously. After 30 days and 45 days, the rats were inoculated with the same method, and Lennon score ≥ 1 was used as the model rats. (2)Grouping and treatment:In addition to the blank group, the successfully modeling rats were randomly divided into a model group, a prednisone group, a Radix Puerariae group, a Geqi group, a Geshen group, a Gemiao group and a Geqishenmiao group, with 20 rats in each group. After 2 weeks of successful modeling, the blank group and model group were given distilled water by gavage, and the other groups were given drugs by gavage for 4 weeks. (3)Sample detection:The mitochondrial ultrastructure of skeletal muscle was observed by transmission electron microscope. The expression of FGF, NGF and IGFs was detected by immunohistochemistry. Results Compared with the model group, the mitochondrial morphology of each group recovered, and the whole morphology of the Geqi group and the Geqishenmiao group recovered better after treatment; Compared with the model group, the positive expression rates of FGF, NGF and IGFs in each treatment group increased, and the difference was statistically significant (P<0.01). Conclusion Radix Puerariae and its compatibility may promote the recovery of mitochondrial structure by improving the positive expression of myogenic regulatory factors, so as to achieve the purpose of treating myasthenia gravis. |
| Key words: myasthenia gravis compatibility of Radix Puerariae ultrastructure of skeletal muscle mitochondria regulation factors of muscle formation |
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