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李翔,覃艮艳,彭俊,欧阳云,蒋鹏飞,彭清华.密蒙花颗粒剂对去势雄兔泪腺组织细胞Caspase-1和IL-18的影响[J].湖南中医药大学学报,2019,39(12):1440-1443[点击复制] |
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| 密蒙花颗粒剂对去势雄兔泪腺组织细胞Caspase-1和IL-18的影响 |
| 李翔,覃艮艳,彭俊,欧阳云,蒋鹏飞,彭清华 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学, 湖南 长沙 410208;中医药防治眼耳鼻咽喉疾病湖南省重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察密蒙花颗粒剂对去势雄兔泪腺组织细胞半胱氨酸天冬氨酸蛋白酶1（cysteine aspartic protease-1，Caspase-1）和白细胞介素-18（interleukin-18，IL-18）表达的影响。方法 将30只新西兰长耳白兔随机分为5组，分别为空白组（不灌胃）、模型组（不灌胃）、密蒙花颗粒剂组（灌胃药物为密蒙花颗粒剂）、安慰剂组（灌胃药物为生理盐水）、睾酮组（在兔大腿肌肉处注射丙酸睾酮注射液）。除空白组外，所有兔子的造模方式均为双侧睾丸及附睾切除术。干预30 d后，将所有兔子处死，摘取泪腺组织进行免疫组化染色观察泪腺组织细胞结构并检测泪腺组织中Caspase-1和IL-18的表达。结果 免疫组化染色显示：模型组兔子泪腺组织排列不整齐，细胞破碎；密蒙花颗粒剂组与睾酮组兔子泪腺组织排列整齐，细胞结构清晰。与空白组相比，模型组Caspase-1、IL-18平均光密度差异有统计学意义（P<0.05）。与模型组相比，安慰剂组Caspase-1、IL-18平均光密度差异无统计学意义（P>0.05）；密蒙花颗粒剂组与睾酮组Caspase-1、IL-18平均光密度值降低，差异均有统计学意义（P<0.05）。与睾酮组相比，密蒙花颗粒剂组Caspase-1、IL-18平均光密度差异无统计学意义（P>0.05）。结论 密蒙花颗粒剂对去势雄兔泪腺细胞Caspase-1、IL-18表达具有抑制作用，密蒙花颗粒剂可能通过抑制泪腺组织中Caspase-1、IL-18的表达，减少泪腺组织细胞的焦亡，从而达到治疗干眼的目的。 |
| 关键词: 密蒙花颗粒剂 去势雄兔 泪腺组织 半胱氨酸天冬氨酸蛋白酶1 白细胞介素-18 |
| DOI：10.3969/j.issn.1674-070X.2019.12.004 |
| 投稿时间：2019-09-02 |
| 基金项目:国家自然科学基金面上资助项目（30772824，81574031）；中医药防治五官科疾病湖南省重点实验室建设项目（2017TP1018）；长沙市科技计划项目（K1501014-31，kc1704005）；中央财政支持地方高校建设项目；国家中医药管理局中医眼科学重点学科建设项目；湖南省中医五官科学重点学科建设项目；湖南省研究生科研创新项目（CX2018B497）。 |
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| Effects of Mimenghua Granules on Caspase-1 and IL-18 of Cells from Lacrimal Gland Tissue in Castrated Male Rabbits |
| LI Xiang,QIN Genyan,PENG Jun,OUYANG Yun,JIANG Pengfei,PENG Qinghua |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases in Hunan Province, Changsha, Hunan 410208, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases in Hunan Province, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of Mimenghua Granules on the expression of cysteine aspartic protease-1 (Caspase-1) and interleukin-18 (IL-18) in cells from lacrimal gland tissue of castrated male rabbits. Methods A total of 30 New Zealand long-eared white rabbits were randomly divided into 5 groups:a blank group (no gavage), a model group (no gavage), Mimenghua Granules group (Mimenghua Granules as the gavage drugs), a placebo group (saline as the gavage drugs), a testosterone group (testosterone propionate injection was injected into the rabbit thigh muscle). Except for the blank group, all rabbits were subjected to bilateral testicular and epididymal resection for molding. After 30 days of intervention, all rabbits were sacrificed, and lacrimal gland tissue was harvested for immunohistochemical staining to observe the structure of lacrimal gland tissue. And the expression of 2 pyroptosis factors of Caspase-1 and IL-18 in lacrimal gland tissue was detected. Results Immunohistochemistry staining showed that the lacrimal gland tissue of the model group was not arranged neatly and the cells were broken; the lacrimal gland tissue of the Mimenghua Granules group and the testosterone group were arranged neatly and the cell structure was clear. Compared with the blank group, the difference of average optical density of Caspase-1 and IL-18 in the model group was statistically significant (P<0.05). Compared with the model group, there was no statistically significant difference in the average optical of Caspase-1 and IL-18 in the placebo group (P>0.05); while the differences in average optical density of Caspase-1 and IL-18 in the Mimenghua Granules group and the testosterone group were statistically significant (P<0.05). Compared with the testosterone group, the difference of average optical density of Caspase-1 and IL-18 in the Mimenghua Granules group was not statistically significant (P>0.05). Conclusion Mimenghua Granules has an inhibitory effect on the expression of Caspase-1 and IL-18 in castrated male rabbits' lacrimal gland cells. Mimenghua Granules may reduce the pyroptosis of lacrimal gland tissue through inhibiting the expression of Caspase-1 and IL-18 in lacrimal gland tissue to achieve the purpose of treating dry eyes. |
| Key words: Mimenghua Granules castrated male rabbits lacrimal gland tissue Caspase-1 IL-18 |
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