| 引用本文: |
杜俊锋,涂亮星,胡凯莉,奉建芳.葛根素纳米晶的制备工艺及其表征[J].湖南中医药大学学报,2017,37(4):369-372[点击复制] |
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| 葛根素纳米晶的制备工艺及其表征 |
| 杜俊锋,涂亮星,胡凯莉,奉建芳 |
| (上海中医药大学, 上海 201203;上海中医药大学, 上海 201203;桂林医学院, 广西 桂林 451004) |
| 摘要: |
| 目的 研究葛根素纳米晶的制剂处方、制备工艺及其对粒径的影响，并对葛根素纳米晶进行表征。方法 采用高压均质法制备葛根素纳米晶，以粒径为指标，考察稳定剂、药辅比、投料量、制备温度等参数对纳米晶制备的影响。在此基础上，考察干燥工艺对葛根素纳米晶固化的影响。采用扫描电镜（SEM）及透射电镜（TEM）进行葛根素及纳米结晶形态学的观察。结果 采用聚维酮K30作为稳定剂、药辅比为1：2、制备温度43 ℃以上时，可制得粒径为20～50 nm的葛根素纳米晶。投药量对纳米晶粒径没有显著性影响，投药量为10%（w/v）时，采用700 bar 5次的高压均质条件即可制得所需的纳米晶。冷冻干燥可以用于纳米晶的干燥。将葛根素制成纳米结晶之后，其外观由不规则状态变为圆整的球形。结论 所确定的葛根素纳米晶制剂处方与制备工艺条件可行，可有效地制备100 nm以下的葛根素纳米晶。 |
| 关键词: 葛根素 纳米晶 制备工艺 粒径 表征 |
| DOI：10.3969/j.issn.1674-070X.2017.04.006 |
| 投稿时间：2016-11-21 |
| 基金项目:国家“973”计划项目（2009CB930300）；上海市优秀技术带头人计划项目（13XD1425300）；上海市科委纳米专项项目（11nm0506700） |
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| Characteristics and Process Technology of Peurarin Nanocrystals |
| DU Junfeng,TU Liangxing,HU Kaili,FENG Jianfang |
| (Shanghai University of Chinese Medicine, Shanghai 201203, China;Shanghai University of Chinese Medicine, Shanghai 201203, China;Guilin Medical University, Guilin, Guangxi 451004, China) |
| Abstract: |
| Objective In this study, the production, process technology and effects of particle size of puerarin nanocrystals were investigated, and the puerarin nanocrystals were characterized. Methods High pressure homogenization (HPH) was used for formula and production studies. Particle size as an indicator, we studied the effects of stabilizers, drug/stabilizer ration, drug input and production temperature on the production of peurarin nanocrystals, and then drying method were introduced to study the solidification of nanocrystals. We introduced scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for morphologic observation for crystalline study. Results In the production study, puerarin nanocrystal with diameter of 20～50 nm could be produced after using PVP K30 as stabilizer, with drug/stabilizer ratio 1:2, temperature above 43℃. The amounts of puerarin input has no significant influence on nanocrystals production. In poilet scaled experiments, nanocrystals with puerarin input of 10% (w/v) were produced at 700 bar for 5 cycles, and the nanocrystals prepared were solidified by freezing drying and spray drying. We found that after transferred puerarin into nanocrystals, the irregular shape for puerarin was changed to spherical shape for nanocrystals. Conclusion The formula and production processes estabilished were reliable and repeatability, the puerarin nanocrystals with diameter below 100 nm could be prepared effectively. |
| Key words: puerarin nanocrystals process technology particle size superficial characteristics |
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