| 引用本文: |
吴世婷,刘丽芳,熊家青,丁玲,曾叶,肖洒.黄芪解毒汤诱导乳腺癌细胞凋亡及对β-catenin、C-myc基因表达影响的研究[J].湖南中医药大学学报,2017,37(4):357-360[点击复制] |
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| 黄芪解毒汤诱导乳腺癌细胞凋亡及对β-catenin、C-myc基因表达影响的研究 |
| 吴世婷,刘丽芳,熊家青,丁玲,曾叶,肖洒 |
| (湖南中医药大学第一附属医院乳腺外科, 湖南 长沙 410007;湖南中医药大学研究生院, 湖南 长沙 410208;湖南航天医院, 湖南 长沙 410205) |
| 摘要: |
| 目的 探讨黄芪解毒汤体外干预人乳腺癌细胞MCF-7凋亡及对β-catenin、C-myc基因表达的影响，及该方抑制乳腺癌复发转移的可能机制。方法 培养MCF-7细胞并将其分为黄芪解毒汤组、阿霉素组及空白对照组。以黄芪解毒汤浓缩液、阿霉素液及细胞培养液分别干预各组MCF-7细胞24h后，以流式细胞仪检测其对MCF-7乳腺癌细胞凋亡的影响，实时荧光定量PCR检测其对β-catenin， C-myc基因表达的影响。结果 与空白对照组比较，黄芪解毒汤组、阿霉素组可明显诱导MCF-7细胞的凋亡（P<0.01）；黄芪解毒汤组及阿霉素组β-catenin、C-myc表达量明显低于空白对照组（P<0.01）。结论 黄芪解毒可诱导乳腺癌细胞MCF-7凋亡，显著降低β-catenin、 C-myc基因表达，该结果为黄芪解毒汤在乳腺癌术后治疗中的应用提供实验依据。 |
| 关键词: MCF-7 β-catenin，C-myc 细胞凋亡 黄芪解毒汤 |
| DOI：10.3969/j.issn.1674-070X.2017.04.003 |
| 投稿时间：2016-08-02 |
| 基金项目:湖南省科技厅（2012SK3137）；湖南省教育厅（13A071） |
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| Effects of Huangqi Jiedu Decoction on Inducing Apoptosis of Breast Cancer Cells and Gene Expression of β-catenin and C-myc |
| WU Shiting,LIU Lifang,XIONG Jiaqing,DING Ling,ZENG Ye,XIAO Sa |
| (Department of Breast Cancer, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Aerospace Hospital, Changsha, Hunan 410205, China) |
| Abstract: |
| Objective To observe the effects of Huangqi Jiedu decoction on the inducing apoptosis of MCF-7 and expression of β-catenin, C-myc of human breast cancer cells in vitro and to reveal its mechanism of inhibiting recurrence and metastasis of breast cancer. Methods The cultured MCF-7 cells were assigned into Huangqi Jiedu decoction group, doxorubicin group and control group. After intervention for 24 h by Huangqi Jiedu decoction, doxorubicin and cell cultured filed, the apoptosis of breast cancer cell MCF-7 was determined by flow cytometryt. The expression of β-catenin, C-myc genes was detected by Real Time Quantitative-PCR. Results Compared with the control group, Huangqi Jiedu decoction and doxorubicin groups had the more obvious effect on inducing the apoptosis of breast cancer cell MCF-7 (P<0.01). The expressions of β-catenin and C-myc protein in Huangqi Jiedu decoction and doxorubicin groups were significantly lower than those in control group (P<0.01). Conclusion Huangqi Jiedu decoction could induce the apoptosis of MCF-7 breast cancer cell, and significantly prohibit the expression of β-catenin and C-myc genes, which provide the experimental evidence for Huangqijiedu decoction in the treatment for postoperation breast cancer. |
| Key words: MCF-7 β-catenin C-myc apoptosis Huangqi Jiedu decoction |
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