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杨琼梁,欧阳婷,杨仁义,张桃伏,吕琦,颜红.市售不同产地辛夷中挥发油成分分析及木兰脂素的含量测定[J].湖南中医药大学学报,2016,36(10):39-44[点击复制] |
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| 市售不同产地辛夷中挥发油成分分析及木兰脂素的含量测定 |
| 杨琼梁,欧阳婷,杨仁义,张桃伏,吕琦,颜红 |
| (湖南中医药大学药学院, 湖南 长沙 410208;湖南中医药大学中西医结合学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 分析比较市售湖南、广西两个不同产地辛夷中挥发油的化学成分，并对其木兰脂素的含量进行测定。方法 采用GC-MS联用技术对湖南、广西两个产地的辛夷挥发油成分进行分析，采用HPLC对两个产地辛夷中木兰脂素含量进行测定。结果 湖南、广西产地的辛夷挥发油分别分离鉴定出62和64种化合物，主要成分为桉油精、β-蒎烯、2-茨酮、香叶基芳樟醇、α-松油醇等。采用HPLC测定辛夷中木兰脂素的含量，结果显示，木兰脂素在2~256 μg/mL浓度范围内与峰面积呈良好线性关系（r=0.999 96，n=6），平均加样回收率为100.32%，RSD为0.04%（n=6）。木兰脂素含量为：湖南产地（0.87±0.05）%、广西产地（0.81±0.02）%。结论 GC-MS法能快速、简便、高效地检测辛夷挥发油的化学成分，HPLC简便可靠、重复性好，可用于辛夷中木兰脂素的含量测定。 |
| 关键词: 辛夷 GC-MS HPLC 挥发油 木兰脂素 含量测定 |
| DOI：10.3969/j.issn.1674-070X.2016.10.010 |
| 投稿时间：2016-05-09 |
| 基金项目:湖南省大学生研究性学习和创新性实验计划项目（湘教通[2016]283号）；湖南省“中药学”重点学科项目（湘教通[2011]76号）。 |
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| Component Analysis of Volatile Oil of Flos Magnoliae and Assay of Magnolin Content from Different Producing Areas |
| YANG Qiongliang,OUYANG Ting,YANG Renyi,ZHANG Taofu,LV Qi,YAN Hong |
| (School of pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To study the chemical component of volatile oil of Flos Magnoliae from Hunan and Guangxi,and the magnolin content was determined.Methods The volatile oil of the Flos Magnoliae in Hunan and Guangxi was analyzed by GC-MS,and the content of magnolin was determined by HPLC.Results 63 and 65 compounds were respectively separated and identified from Flos Magnoliae volatile oil that was collected from Hunan and Guangxi,mainly including eucalyptol,beta pinene,2-camphor,geranyl linalool,alpha terpineol etc.Magnolin presented a good linearity with peak area between the concentration of 2-256 μg/mL (r=0.99996,n=6).The average recovery was 100.32% with the RSD of 0.04%.The content of Magnolia from the province of Hunan and Guangxi was 0.87%±0.05% and 0.81%±0.02%.Conclusion GC-MS can detect the composition of essential oil quickly and effectively.The analysis of magnolin with HPLC was simple,reliable and reproducible. |
| Key words: Flos Magnoliae GC-MS HPLC volatile oil magnolin content assaying |
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